Haddad Faten, Bousselmi Jihène, Mrabet Ali, Ben Fadhel Kamel
Tunis Med. 2019 Nov;97(11):1224-1228.
Health care-associated infections are a real public health problem. Contaminated medical equipments such as stethoscopes are often an overlooked vector. In our study, we were interested in proving our doctors stethoscopes contamination and in studying the microbiological profile of isolated germs.
This was about a cross-sectional study that lasted 2 months (May and June 2014) including 39 personal stethoscopes of all grade doctors working in 8 different departments in Habib Thameur Hospital. The swabs were taken from the membranes of the stethoscopes and sent quickly to our bacteriology service. Then, the bacteriological samples were subcultivated on blood agar. The reading tooks place 24 hours later. Were considered positive the cultures that contained more than 104 colony forming units/ml. Then we proceeded to the identification of the germ.
Fifteen samples from 39 were positive that was 38 %. The coagulase- negative Staphylococcus was the predominant germ. The pathogenic germs were found in 5 positive samples that was 12 % from all the samples. They were distributed as follows: 2 Methicillin-susceptible Staphylococci Aureus, 2 methicillin-resistant coagulase- negative Staphylococci and one Pseudomonas aerogenusa. The pathogenic germs were found in stethoscopes taken from cardiology and intensive care unit (ICU). In cardiology, 7/9 stethoscopes were positive (5 coagulase- negative Staphylococcus among them 2 resistant methicillin and 2 Staphylococcus aureus.) In ICU, 6/13 stethoscopes were positive among them one Pseudomonas aerogenusa.
The stethoscopes constitute bacterial reservoirs. It is necessary to perform a procedure for the good practices of disinfection.
医疗保健相关感染是一个切实存在的公共卫生问题。诸如听诊器等受污染的医疗设备往往是一个被忽视的传播媒介。在我们的研究中，我们旨在证实我们医生的听诊器受到污染，并研究分离出的细菌的微生物学特征。
这是一项为期2个月（2014年5月和6月）的横断面研究，纳入了哈比卜·塔穆尔医院8个不同科室所有级别医生的39个个人听诊器。从听诊器的膜片上采集拭子，并迅速送至我们的细菌学实验室。然后，将细菌学样本接种在血琼脂上进行亚培养。24小时后进行读数。培养物中菌落形成单位每毫升超过104个的被视为阳性。然后我们进行细菌鉴定。
39个样本中有15个呈阳性，即38%。凝固酶阴性葡萄球菌是主要细菌。在5个阳性样本中发现了致病细菌，占所有样本的12%。它们的分布如下：2株甲氧西林敏感金黄色葡萄球菌、2株耐甲氧西林凝固酶阴性葡萄球菌和1株铜绿假单胞菌。在从心脏病科和重症监护病房（ICU）采集的听诊器中发现了致病细菌。在心脏病科，9个听诊器中有7个呈阳性（其中5个是凝固酶阴性葡萄球菌，2个是耐甲氧西林的，2个是金黄色葡萄球菌）。在ICU，13个听诊器中有6个呈阳性，其中1个是铜绿假单胞菌。
听诊器构成了细菌储存库。有必要实施良好的消毒程序。
