Guo Qingsheng, Wang Yao, Chen Cang, Wei Dan, Fu Jianping, Xu Hong, Gu Hongchen
Shanghai Jiao Tong University Affiliated Sixth Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, P. R. China.
Department of Mechanical Engineering, Department of Biomedical Engineering, Department of Cell and Developmental Biology, University of Michiga Ann Arbor, Ann Arbor, MI, 48109, USA.
Small. 2020 Apr;16(17):e1907521. doi: 10.1002/smll.201907521. Epub 2020 Mar 16.
The development of a powerful immunoassay platform with capacities of both simplicity and high multiplexing is promising for disease diagnosis. To meet this urgent need, for the first time, a multiplexed luminescent oxygen channeling immunoassay (multi-LOCI) platform by implementation of LOCI with suspension array technology is reported. As the microcarrier of the platform, a unique dual-functional barcode with a host-guest structure composed of a quantum dot host bead (QDH) and LOCI acceptor beads (ABs) is designed, in which QDH provides function of high coding capacity while ABs facilitate the LOCI function. The analytes bridge QDH@ABs and LOCI donor beads (DBs) into a close proximity, forming a QDH@ABs-DBs "host-guest-satellite" superstructure that generates both barcode signal from QDH and LOCI signal induced by singlet oxygen channeling between ABs and DBs. Through imaging-based decoding, different barcodes are automatically distinguished and colocalized with LOCI signals. Importantly, the assay achieves simultaneous detection of multiple analytes within one reaction, simply by following a "mix-and-measure" protocol without the need for tedious washing steps. Furthermore, the multi-LOCI platform is validated for real sample measurements. With the advantages of robustness, simplicity, and high multiplexing, the platform holds great potential for the development of point-of-care diagnostics.
开发一种兼具简单性和高多重检测能力的强大免疫分析平台对疾病诊断具有重要意义。为满足这一迫切需求，首次报道了一种通过将发光氧通道免疫分析（LOCI）与悬浮阵列技术相结合的多重发光氧通道免疫分析（multi-LOCI）平台。作为该平台的微载体，设计了一种独特的具有主客体结构的双功能条形码，它由量子点主体珠（QDH）和LOCI受体珠（AB）组成，其中QDH提供高编码能力的功能，而AB则促进LOCI功能。分析物将QDH@AB与LOCI供体珠（DB）拉近，形成QDH@AB-DB“主客体-卫星”超结构，该结构既产生来自QDH的条形码信号，又产生由AB与DB之间的单线态氧通道诱导的LOCI信号。通过基于成像的解码，可以自动区分不同的条形码并将其与LOCI信号共定位。重要的是，该分析方法只需遵循“混合-测量”方案，无需繁琐的洗涤步骤，即可在一个反应中同时检测多种分析物。此外，multi-LOCI平台已通过实际样品测量验证。该平台具有稳健性、简单性和高多重检测的优点，在即时诊断的发展中具有巨大潜力。
