Department of Biological Sciences, College of Science, Clemson University, Clemson, SC, USA; Graduate Program in Environmental Toxicology, Clemson University, Clemson, SC, USA.
Graduate Program in Environmental Toxicology, Clemson University, Clemson, SC, USA.
Mar Environ Res. 2020 Apr;156:104915. doi: 10.1016/j.marenvres.2020.104915. Epub 2020 Feb 8.
With oil spills, and other sources of aromatic hydrocarbons, being a continuous threat to coral reef systems, and most reef fish species being protected or difficult to collect, the use of the invasive lionfish (Pterois volitans) might be a good model species to monitor biomarkers in potentially exposed fish in the Caribbean and western Atlantic. The rapid expansion of lionfish in the Caribbean and western Atlantic, and the unregulated fishing for this species, would make the lionfish a suitable candidate as biomonitoring species for oil pollution effects. However, to date little has been published about the responses of lionfish to environmental pollutants. For this study lionfish were collected in the Florida Keys a few weeks after Hurricane Irma, which sank numerous boats resulting in leaks of oil and fuel, and during the winter and early spring after that. Several biomarkers indicative of exposure to PAHs (bile fluorescence, cytochrome P450-1A induction, glutathione S-transferase activity) were measured. To establish if these biomarkers are inducible in PAH exposed lionfish, dosing experiments with different concentrations of High Energy Water Accommodated Fraction of crude oil were performed. The results revealed no significant effects in the biomarkers in the field collected fish, while the exposure experiments demonstrated that lionfish did show strong effects in the measured biomarkers, even at the lowest concentration tested (0.3% HEWAF, or 25 μg/l ƩPAH50). Based on its widespread distribution, relative ease of collection, and significant biomarker responses in the controlled dosing experiment, it is concluded that lionfish has good potential to be used as a standardized biomonitoring species for oil pollution in its neotropical realm.
随着溢油和其他芳香烃源不断威胁珊瑚礁系统，而且大多数珊瑚礁鱼类受到保护或难以采集，因此，使用入侵的狮子鱼（Pterois volitans）可能是监测加勒比海和西大西洋中潜在暴露鱼类生物标志物的良好模式物种。狮子鱼在加勒比海和西大西洋的迅速扩张以及对这种鱼类的无管制捕捞，将使狮子鱼成为监测石油污染影响的合适候选生物监测物种。然而，迄今为止，关于狮子鱼对环境污染物的反应的研究很少。在这项研究中，狮子鱼是在飓风艾玛（Irma）过后的几周内在佛罗里达群岛收集的，这次飓风导致许多船只沉没，造成石油和燃料泄漏，并且在那之后的冬季和早春也进行了收集。测量了几种表明接触多环芳烃（胆汁荧光，细胞色素 P450-1A 诱导，谷胱甘肽 S-转移酶活性）的生物标志物。为了确定这些生物标志物是否可诱导接触多环芳烃的狮子鱼，进行了不同浓度的高能水容纳的原油馏分的剂量实验。结果表明，现场采集的鱼类中的生物标志物没有明显影响，而暴露实验表明，即使在测试的最低浓度（0.3％HEWAF，或 25μg/lƩPAH50）下，狮子鱼在测量的生物标志物中也表现出强烈的影响。基于其广泛的分布，相对容易收集以及在受控剂量实验中的显著生物标志物反应，得出的结论是，狮子鱼具有成为其新热带领域中用于石油污染标准化生物监测物种的良好潜力。
