State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, PR China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, PR China.
Dev Comp Immunol. 2020 Jul;108:103673. doi: 10.1016/j.dci.2020.103673. Epub 2020 Mar 7.
Fas-associated death domain (FADD) is an adaptor protein that functions in transferring the apoptotic signals regulated by the death receptors. In this study, a full-length cDNA of FADD homologue in sea cucumber Apostichopus japonicas (AjFADD) was cloned and characterized, and its functional roles in apoptosis investigated. In healthy sea cucumbers, AjFADD was expressed in all detected tissues, with higher levels in coelomocytes and intestine. AjFADD mRNA and protein levels were significantly expressed in coelomocytes after exposed with LPS or poly (I:C) in vitro, and challenged with Vibrio splendidus in vivo. Moreover, siRNA-mediated AjFADD knockdown in coelomocyte much decreased AjFADD mRNA and protein levels as well as the coelomocytes apoptosis levels. Furthermore, over-expression of the expression plasmid pcDNA3.1 encoding AjFADD (pcAjFADD) significantly increased the apoptosis levels in HEK293 cells. Taken together, our results support that AjFADD is a novel pro-apoptotic protein that might play key roles in defensing the bacterial and virus invasion in sea cucumber.
Fas 相关死亡结构域（FADD）是一种衔接蛋白，在传递受死亡受体调控的凋亡信号中发挥作用。在这项研究中，我们克隆并鉴定了海参（Apostichopus japonicas）FADD 同源物的全长 cDNA，并研究了其在凋亡中的功能作用。在健康海参中，AjFADD 在所有检测到的组织中表达，在体腔细胞和肠中表达水平更高。AjFADD mRNA 和蛋白水平在体外用 LPS 或 poly（I:C）处理以及体内用灿烂弧菌攻毒后，在体腔细胞中显著表达。此外，siRNA 介导的 AjFADD 在体腔细胞中的敲低显著降低了 AjFADD mRNA 和蛋白水平以及体腔细胞的凋亡水平。此外，表达质粒 pcDNA3.1 编码 AjFADD（pcAjFADD）的过表达显著增加了 HEK293 细胞的凋亡水平。总之，我们的结果支持 AjFADD 是一种新型的促凋亡蛋白，可能在海参抵御细菌和病毒入侵中发挥关键作用。
