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利用微卫星标记对马尔霍兹山羊品种进行遗传特征分析。

Genetic characterization of Markhoz goat breed using microsatellite markers.

作者信息

Asroush Fariborz, Mirhoseini Seyed-Ziaeddin, Badbarin Nejat, Seidavi Alireza, Tufarelli Vincenzo, Laudadio Vito, Dario Cataldo, Selvaggi Maria

机构信息

Department of Animal Science, Rasht Branch, Islamic Azad University, Rasht, Iran.

Department of Animal Science, University of Guilan, Rasht, Iran.

出版信息

Arch Anim Breed. 2018 Dec 6;61(4):469-473. doi: 10.5194/aab-61-469-2018. eCollection 2018.

Abstract

The use of molecular markers can support the management of endangered populations and should be combined with appropriate breeding strategies to improve productive traits avoiding the decline of the breed. The genetic variability at 10 microsatellite loci were investigated in a sample of 100 unrelated Markhoz goats (77 females and 23 males). The investigated population was reared at the Sanandaj Markhoz goat Performance Testing Station in Kurdistan, Iran. Markhoz goat, a multipurpose breed, is one of the most valuable genetic resources in Iran. All the studied loci were found to be polymorphic and a total number of 52 alleles were identified with an average number of alleles of 5.2. Moreover, some population genetic indices, such as observed and expected heterozygosity, observed and expected number of alleles, Shannon's index, Nei's expected heterozygosity, and polymorphism information content were also calculated. Despite the decreasing population size, Markhoz goat genetic diversity is still conserved. The breed seems to have a good level of genetic variability and, as a consequence, a potential margin of adaptability to environment and for future genetic improvement.

摘要

分子标记的使用有助于濒危种群的管理,应与适当的育种策略相结合,以改善生产性状,避免品种衰退。在100只无亲缘关系的马尔霍兹山羊(77只雌性和23只雄性)样本中,研究了10个微卫星位点的遗传变异。所研究的种群在伊朗库尔德斯坦的萨南达杰马尔霍兹山羊性能测试站饲养。马尔霍兹山羊是一种多用途品种,是伊朗最有价值的遗传资源之一。所有研究位点均为多态性,共鉴定出52个等位基因,平均等位基因数为5.2。此外,还计算了一些群体遗传指标,如观察到的和预期的杂合度、观察到的和预期的等位基因数、香农指数、内氏预期杂合度和多态信息含量。尽管种群规模在减小,但马尔霍兹山羊的遗传多样性仍得到了保留。该品种似乎具有良好的遗传变异性,因此具有适应环境和未来遗传改良的潜在空间。

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