长链非编码 RNA MEG3 中的遗传变异与乳腺癌易感性及埃及人群血清 MEG3 表达水平的关联分析。

Association analyses of a genetic variant in long non-coding RNA MEG3 with breast cancer susceptibility and serum MEG3 expression level in the Egyptian population.

机构信息

Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, Fayoum, Egypt.

Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.

出版信息

Cancer Biomark. 2020;28(1):49-63. doi: 10.3233/CBM-191072.

DOI:10.3233/CBM-191072

PMID:32176630

Abstract

BACKGROUND

LncRNA MEG3 rs7158663 has been shown to confer cancer susceptibility, maybe through altering its gene expression level.

OBJECTIVE

We aimed to weigh the effect of rs7158663 on MEG3 serum level and breast cancer susceptibility.

METHODS

We genotyped rs7158663 G > A and measured serum MEG3 in 150 breast cancer, 95 fibroadenoma , and 154 controls by the TaqMan method.

RESULTS

The presence of rs7158663 G > A is a risk factor for breast cancer among fibroadenoma patients and controls, AA vs. GG genotypes (OR = 6.320, 95% CI = 2.587-15.439, P< 0.0001 when compared to controls and OR = 10.825, 95% CI = 1.929-60.742, P= 0.007 when compared to fibroadenoma). Decreased serum MEG3 was observed in breast cancer group when compared with fibroadenoma and/or controls [median (IQR) = 0.43 (0.27-0.55)] (P< 0.0001). However, increased serum MEG3 was noted in fibroadenoma group when compared with controls (P< 0.0001). A significance decreased serum MEG3 was found to be associated with mutant A allele than with wild G allele (P< 0.0001). The results showed that rs7158663 and lower MEG3 were significantly associated with patients with higher TNM staging and larger tumor size > 5 cm.

CONCLUSION

The presence of both rs7158663 and low MEG3 are diagnostic and unfavorable prognostic factors for BC patients.

摘要

背景

长链非编码 RNA MEG3 的 rs7158663 已被证明可导致癌症易感性，可能是通过改变其基因表达水平。

目的

我们旨在衡量 rs7158663 对 MEG3 血清水平和乳腺癌易感性的影响。

方法

我们通过 TaqMan 法对 150 例乳腺癌、95 例纤维腺瘤和 154 例对照进行 rs7158663 G > A 基因分型和测量血清 MEG3。

结果

rs7158663 G > A 的存在是纤维腺瘤患者和对照者乳腺癌的危险因素，AA 与 GG 基因型相比（OR = 6.320，95%CI = 2.587-15.439，与对照组相比 P < 0.0001；OR = 10.825，95%CI = 1.929-60.742，与纤维腺瘤相比 P = 0.007）。与纤维腺瘤和/或对照相比，乳腺癌组血清 MEG3 降低[中位数（IQR）= 0.43（0.27-0.55）]（P < 0.0001）。然而，与对照组相比，纤维腺瘤组血清 MEG3 增加（P < 0.0001）。与野生 G 等位基因相比，突变 A 等位基因与血清 MEG3 降低显著相关（P < 0.0001）。结果表明，rs7158663 和低 MEG3 与 TNM 分期较高和肿瘤大小> 5cm 的患者显著相关。