Zapata A, Hernández-García J M, de la Fuente P
Biochemistry Service, La Paz Hospital, Madrid, Spain.
Scand J Clin Lab Invest. 1988 Feb;48(1):39-43. doi: 10.3109/00365518809085392.
An analysis was made of samples of amniotic fluid from 82 pregnant women with normal pregnancies, ranging from 29 to 40 weeks of amenorrhoea. Lipid extraction was quantitative and individual phospholipids were isolated by two-dimensional chromatography. The lecithin/sphingomyelin ratio (L/S) increased significantly (t = 2.17; p less than 0.05) between 35 and 36 weeks of amenorrhoea (from 2.9 +/- 1.0 to 4.3 +/- 1.7). Phosphatidyl-glycerol (PG) was detected from 35 weeks of pregnancy, the time at which the incidence of amniotic samples with mature surfactant (L/S greater than or equal to 2.7 and presence of PG) was 9%; mature surfactant incidence increased to 55.5% at 36 weeks and 100% at 37 or more weeks. There was a good correlation between surfactant levels in amniotic fluid and new-born respiratory function.
对82名妊娠正常的孕妇的羊水样本进行了分析，这些孕妇的闭经周数在29至40周之间。脂质提取是定量的，通过二维色谱法分离出各个磷脂。在闭经35至36周之间，卵磷脂/鞘磷脂比值（L/S）显著增加（t = 2.17；p < 0.05）（从2.9±1.0增至4.3±1.7）。妊娠35周时检测到磷脂酰甘油（PG），此时具有成熟表面活性剂的羊水样本发生率（L/S大于或等于2.7且存在PG）为9%；成熟表面活性剂发生率在36周时增至55.5%，在37周及以上时为100%。羊水表面活性剂水平与新生儿呼吸功能之间存在良好的相关性。
