Al-Khwarizmi College of Engineering, University of Baghdad, Baghdad,, Iraq.
Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia, Johor Bahru, Malaysia.
Recent Pat Food Nutr Agric. 2020;11(3):211-218. doi: 10.2174/2212798411666200316153148.
For many years, Ganoderma was highly considered as biofactory for the production of different types of bioactive metabolites. Of these bioactive compounds, polysaccharides gained much attention based on their high biotherapeutic properties. Therefore, special attention has been paid during the last years for the production of mushrooms bioactive compounds in a closed cultivation system to shorten the cultivation time and increase the product yield.
This work focuses on the development of a simple cultivation strategy for exopolysaccharides (EPS) production using Ganoderma lucidum and submerged cultivation system.
At first, the best medium supporting EPS production was chosen experimentally from the current published data. Second, like many EPS production processes, carbon and nitrogen concentrations were optimized to support the highest production of polysaccharides in the shake flask level. Furthermore, the process was scaled up in 16-L stirred tank bioreactor.
The results clearly demonstrated that the best cultivation strategy was cultivation under controlled pH conditions (pH 5.5). Under this condition, the maximal volumetric and specific yield of EPS production were, 5.0 g/L and 0.42 g/g, respectively.
The current results clearly demonstrate the high potential use of submerged cultivation system as an alternative to conventional solid-state fermentation for EPS production by G. lucidum. Furthermore, the optimization of both carbon and nitrogen sources concentration and scaling up of the process showed a significant increase in both volumetric and specific EPS production.
多年来，灵芝一直被高度视为生产各种生物活性代谢物的生物工厂。在这些生物活性化合物中，多糖因其高生物治疗特性而备受关注。因此，近年来，人们特别关注在封闭培养系统中生产蘑菇生物活性化合物，以缩短培养时间并提高产品产量。
本工作重点开发一种使用灵芝和浸没式培养系统生产胞外多糖（EPS）的简单培养策略。
首先，从当前已发表的数据中通过实验选择最适合 EPS 生产的培养基。其次，与许多 EPS 生产工艺一样，优化碳氮浓度以支持在摇瓶水平上生产最高产量的多糖。此外，该工艺在 16-L 搅拌罐生物反应器中进行放大。
结果清楚地表明，最佳的培养策略是在控制 pH 值条件（pH 5.5）下进行培养。在此条件下，EPS 生产的最大体积和比产率分别为 5.0 g/L 和 0.42 g/g。
目前的结果清楚地表明，浸没式培养系统作为灵芝 EPS 生产的替代传统固态发酵具有很高的潜力。此外，对碳氮源浓度的优化和工艺放大显示出体积和比 EPS 生产均显著增加。
