大鼠体内3α-羟基-5β-胆甾-6-烯-24-酸还原为石胆酸的过程

Reduction of 3 alpha-hydroxy-5 beta-chol-6-en-24-oic acid to lithocholic acid in rats.

作者信息

Kimura K, Ogura M

机构信息

Department of Biochemistry, Tottori University School of Medicine, Japan.

出版信息

Steroids. 1988 Mar-Apr;51(3-4):337-48. doi: 10.1016/0039-128x(88)90023-2.

DOI:10.1016/0039-128x(88)90023-2

PMID:3217958

Abstract

After [24-14C]delta 6-lithocholic acid was injected into the cecum of rats, [14C]lithocholic acid was identified as a metabolite in feces. When the labeled delta 6-bile acid was injected intraperitoneally into bile-fistula rats, radioactivity excreted in bile was contained most abundantly in the taurine-conjugated fraction of bile acids. In the fraction, taurine conjugate of [14C]delta 6-lithocholic acid but of neither [14C]lithocholic acid nor other bile acids was found. The results showed that [24-14C]delta 6-lithocholic acid was reduced to [14C]lithocholic acid by the intestinal flora but not by the liver, which, however, was capable of conjugating delta 6-lithocholic acid with taurine.

摘要

将[24 - 14C]δ6 - 石胆酸注入大鼠盲肠后，[14C]石胆酸被鉴定为粪便中的一种代谢产物。当将标记的δ6 - 胆汁酸腹腔注射到胆瘘大鼠体内时，胆汁中排泄的放射性物质在胆汁酸的牛磺酸结合部分中含量最为丰富。在该部分中，发现了[14C]δ6 - 石胆酸的牛磺酸结合物，但未发现[14C]石胆酸或其他胆汁酸的牛磺酸结合物。结果表明，[24 - 14C]δ6 - 石胆酸是通过肠道菌群而非肝脏还原为[14C]石胆酸的，不过肝脏能够使δ6 - 石胆酸与牛磺酸结合。