Frolova E I, Tarunina M V, Zatsepina O V, Poliakov V Iu, Chentsov Iu S
Tsitologiia. 1988 Sep;30(9):1084-90.
The ultrastructure and differential staining of minute chromosomes (MC) from mouse fibroblasts line C3H10T1/2 (LS9/0.1) was studied. MC in mitotic cells of this line contain R-positive material and are not stained according to the G- and C-methods. Reconstruction of ultrathin serial sections made it possible to identify MC in situ. It is shown that the basic structural unit of MC is a DNP fibril 20-30 nm in diameter. The density of packing of the MC material corresponds to that of normal chromosomal arms. In some cases the fibrillar material is present between MC and normal chromosomes. It is suggested therefore that some MC may be structurally linked to a normal chromosome arm. The MC structure is discussed in terms of the ultrastructural properties of R- and C-positive segments.
对小鼠成纤维细胞系C3H10T1/2（LS9/0.1）微小染色体（MC）的超微结构和鉴别染色进行了研究。该细胞系有丝分裂细胞中的MC含有R阳性物质，并且不能根据G法和C法进行染色。超薄连续切片的重建使得在原位鉴定MC成为可能。结果表明，MC的基本结构单位是直径为20 - 30nm的脱氧核糖核蛋白（DNP）纤维。MC物质的堆积密度与正常染色体臂的堆积密度相当。在某些情况下，纤维状物质存在于MC和正常染色体之间。因此有人提出，一些MC在结构上可能与正常染色体臂相连。根据R阳性和C阳性片段的超微结构特性对MC的结构进行了讨论。
