Agricultural Research Service, United States Department of Agriculture, San Joaquin Valley Agricultural Sciences Center, Parlier, CA, USA.
Department of Plant Pathology, South China Agricultural University, Guangzhou, People's Republic of China.
Curr Microbiol. 2020 Aug;77(8):1858-1863. doi: 10.1007/s00284-020-01937-0. Epub 2020 Mar 16.
Xylella fastidiosa is a Gram-negative and nutritionally fastidious bacterial pathogen causing Pierce's disease (PD) of grapevine and other plant diseases. X. fastidiosa strain ATCC 35879 which originated from Florida is the designated type strain for the species and for subsp. fastidiosa. In bacterial taxonomy, type strains preserve the characters of the original descriptions. Whole genome sequence of a type strain not only provides a standard reference for bacterial taxonomy, but also facilitates research in other fields such as population diversity and genome evolution. In this study, the whole genome sequence of strain ATCC 35879 was determined using PacBio RSII format. The ATCC 35879 genome has a circular chromosome of 2,565,504 bp with 2,904 predicted protein coding genes and 55 RNA genes, and a circular plasmid of 41,753 bp. The chromosomal sequence of strain ATCC 35879 was compared to that of X. fastidosa subsp. fastidiosa strain M23 from California which causes both PD and almond leaf scorch disease. Genome rearrangements involving a ~ 1,200 K bp region were detected. Genome annotations showed clusters of phage-related genes around the rearrangement junctions, suggesting the likely involvement of phage activities. This is the first report on genome structure variations within strains of X. fastidiosa subsp. fastidiosa.
韧皮部坏死杆菌是一种革兰氏阴性、营养要求苛刻的细菌病原体，可引起葡萄藤皮尔斯病（PD）和其他植物疾病。源自佛罗里达州的韧皮部坏死杆菌菌株 ATCC 35879 是该物种和亚种 fastidiosa 的指定模式株。在细菌分类学中，模式株保留了原始描述的特征。模式株的全基因组序列不仅为细菌分类学提供了标准参考，还有利于其他领域的研究，如种群多样性和基因组进化。在这项研究中，使用 PacBio RSII 格式确定了 ATCC 35879 菌株的全基因组序列。ATCC 35879 基因组有一个 2,565,504 bp 的圆形染色体，预测有 2,904 个蛋白编码基因和 55 个 RNA 基因，还有一个 41,753 bp 的圆形质粒。将菌株 ATCC 35879 的染色体序列与来自加利福尼亚的引起 PD 和杏仁叶枯病的 X. fastidiosa 亚种 fastidiosa 菌株 M23 进行比较。检测到涉及约 1,200 Kb 区域的基因组重排。基因组注释显示，在重排连接处周围有噬菌体相关基因簇，表明噬菌体活动可能参与其中。这是关于 X. fastidiosa 亚种 fastidiosa 菌株内基因组结构变异的首次报道。
