National postdoctoral research station, Haitian Water Group Co., Ltd., Chengdu, 610041, People's Republic of China.
Department of Environmental Science and Engineering, College of Architecture and Environment, Sichuan University, Chengdu, 610065, People's Republic of China.
Environ Sci Pollut Res Int. 2020 May;27(15):18242-18253. doi: 10.1007/s11356-020-08245-x. Epub 2020 Mar 16.
A novel bioflocculant (BW-P3) was produced by a strain of Enterobacter sp. P3 using brewery wastewater as substrate and was further applied to remove the colored substance of fracturing flowback water. The optimum conditions for bioflocculant production were specified by the response surface methodology as COD of brewery wastewater 1487.77 mg/L, glucose 8.94 g/L and initial pH 7.09, under which a bioflocculant yield of 1.274 g/L could be reached. The BW-P3 consists of 79.12% polysaccharides and 15.63% protein. Results show that BW-P3 has a high molecular weight (921 kDa) and contains functional groups (hydroxyl, amino, carbonyl, and acylamino) that likely contribute to flocculation. When using the BW-P3 to flocculate fracturing flowback water, the optimal dosage was 1 g/L BW-P3 with addition of 100 mg/L polymeric aluminum chloride as coagulant aid, and treated under 50 °C at pH 7. Under the optimal condition, the removal rates of chroma and suspended solids (SS) of the fracturing flowback water could reach 85% and 52%, respectively.
一种新型生物絮凝剂（BW-P3）由一株肠杆菌属（Enterobacter sp.）P3 菌株利用啤酒废水作为底物生产，进一步应用于去除压裂返排水中的有色物质。响应面法确定了生物絮凝剂生产的最佳条件为：啤酒废水中 COD 为 1487.77mg/L、葡萄糖 8.94g/L 和初始 pH 值 7.09，在此条件下可达到 1.274g/L 的生物絮凝剂产量。BW-P3 由 79.12%的多糖和 15.63%的蛋白质组成。结果表明，BW-P3 具有高分子量（921kDa），并含有可能有助于絮凝的官能团（羟基、氨基、羰基和酰氨基）。当使用 BW-P3 絮凝压裂返排液时，最佳投加量为 1g/L BW-P3，并添加 100mg/L 聚合氯化铝作为助凝剂，在 50°C、pH 值为 7 下处理。在最佳条件下，压裂返排液的色度和悬浮物（SS）去除率分别可达 85%和 52%。
