Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou, Jiangsu, P. R. China.
Anim Biotechnol. 2021 Dec;32(6):676-682. doi: 10.1080/10495398.2020.1740240. Epub 2020 Mar 17.
Specificity Protein 3 (SP3) is a newly identified regulator of tumor growth and invasiveness in humans. In this study, we identified and characterized the function of duck (). The full-length cDNA sequence of the gene was cloned via rapid amplification of cDNA ends. It contained 2468 nucleotides, including a 111 base pair (bp) 5'-untranslated region (UTR), 215 bp 3'-UTR, and 2142 bp open reading frame (ORF), which encoded a 713 amino acid (AA) strongly conserved with Avian . Tissue specificity analysis demonstrated that was constitutively expressed in the eight tissues tested: liver, spleen, lung, heart, kidney, thymus, breast, and leg; and low expression levels were observed in all tissues, except the spleen and thymus. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis revealed that expression rapidly increased after stimulation with both the hepatitis virus (DHV-1) and polyriboinosinic polyribocytidylic acid (poly(I:C)). However, the expression under these treatments varied in kidney and liver tissues; in the liver, increased significantly at 36 h after the DHV-1 treatment and peaked at 72 h after poly(I:C) stimulation. These results suggested that may play a positive role in immune responses against viral infections in ducks.
特异性蛋白 3 (SP3) 是一种新发现的人类肿瘤生长和侵袭的调节因子。在本研究中，我们鉴定并表征了鸭（）。通过快速扩增 cDNA 末端克隆了基因的全长 cDNA 序列。它包含 2468 个核苷酸，包括 111 个碱基对（bp）5'-非翻译区（UTR）、215bp3'-UTR 和 2142bp 开放阅读框（ORF），编码一个 713 个氨基酸（AA）的强烈保守的禽类。组织特异性分析表明，在测试的 8 种组织中均表达，但在除脾脏和胸腺外的所有组织中表达水平较低。实时定量聚合酶链反应（qRT-PCR）分析显示，在受到肝炎病毒（DHV-1）和聚肌胞苷酸（poly(I:C))刺激后，迅速增加。然而，在肾和肝组织中这些处理下的表达不同；在肝中，DHV-1 处理后 36 小时显著增加，并在 poly(I:C)刺激后 72 小时达到峰值。这些结果表明，可能在鸭对病毒感染的免疫反应中发挥积极作用。
