Xu JinZhong, Liu Xiuhong, Wu Bin, Cao YanZhong
SinoUnison Technology Co. Ltd, No. 10 Xinghuo Road, Nanjing, People's Republic of China.
2Jiangxi Science and Technology Normal University, Nanchang, Jiangxi People's Republic of China.
J Food Sci Technol. 2020 Apr;57(4):1216-1232. doi: 10.1007/s13197-019-04153-2. Epub 2019 Nov 29.
In the current study, we have comprehensively analyzed different kinds of pure honey which was produced in various areas in China according to δC-EA -IRMS (AOAC method 998.12) and δC-LC-IRMS (proposed by the Intertek laboratory in Europe) methods. As for the δC-EA -IRMS method, the study was confirmed that the C4 sugar of all authentic honey samples was qualified. Further inter-laboratory comparison experiments using the δC-LC-IRMS method found that all authentic honey samples had ΔδC (‰) values within the naturally occurring range of ± 1‰ for ΔδC (‰) fru-glu. However, about 70% samples had ΔδC (‰) values outside the range of ± 2.1‰ for ΔδC (‰) max., indicating that a large proportion of pure honey in China can't pass the δC-LC-IRMS test, although these honeys were extracted from unadulterated sources. Based on the present findings, we consider that the δC-LC-IRMS method is not appropriate to reliably detect adulterated honeys with C3 sugars in China.
在本研究中，我们根据δC-EA -IRMS（AOAC方法998.12）和δC-LC-IRMS（由欧洲Intertek实验室提出）方法，全面分析了中国不同地区生产的各类纯蜂蜜。对于δC-EA -IRMS方法，研究证实所有正宗蜂蜜样品的C4糖均合格。使用δC-LC-IRMS方法进行的进一步实验室间比较实验发现，所有正宗蜂蜜样品的ΔδC（‰）fru-glu值在自然发生范围±1‰内。然而，约70%的样品ΔδC（‰）max.值超出±2.1‰范围，这表明中国很大一部分纯蜂蜜虽源自未掺假的来源，但无法通过δC-LC-IRMS测试。基于目前的研究结果，我们认为δC-LC-IRMS方法不适用于可靠检测中国含C3糖的掺假蜂蜜。
