伊朗扎博勒一家转诊医院医护人员鼻腔携带的分子特征
Molecular characteristics of nasal carriage among health care workers at a Referral Hospital in Zabol, Iran.
作者信息
Vaez Hamid, Ghalehnoo Zahra Rashki
机构信息
Department of Microbiology, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran.
出版信息
Pan Afr Med J. 2019 Dec 12;34:196. doi: 10.11604/pamj.2019.34.196.16645. eCollection 2019.
INTRODUCTION
is known as one of the most important hospital pathogens responsible for a wide range of infections. Limited data concerning the prevalence of nasal carriage of and its molecular characteristics are available in Zabol province, Iran. Therefore, the aim of the present study was to determine the prevalence of nasal carriage of and its molecular characteristics isolated from health care workers (HCWs).
METHODS
Totally, 251 nasal swabs were collected from HCWs at a referral hospital, from March to September 2017. Disk diffusion method was used to identify Methicillin-Resistant (MRSA). PCR amplification method was used for the detection of following genes; and SCCmec types.
RESULTS
Of 251 collected swabs, 31 (12.4%) cases were identified as carriers, which 14 (14/31; 45.2%) isolates were MRSA. The most prevalent detected genes were and , with 22.6% and 9.7%, respectively. The most prevalent SCCmec type was SCCmec type IV (28.6%).
CONCLUSION
We found that the prevalence of MRSA nasal carriage is at high level and must be considered as a significant health care problem at the investigated hospital. Strict implementation of infection-control policies and rational use of antibiotics are the main pillars for controlling the spread of at hospital.
引言
已知是导致多种感染的最重要的医院病原体之一。关于伊朗扎博勒省鼻携带情况及其分子特征的有限数据。因此，本研究的目的是确定从医护人员（HCWs）分离出的鼻携带情况及其分子特征。
方法
2017年3月至9月，在一家转诊医院从医护人员中总共采集了251份鼻拭子。采用纸片扩散法鉴定耐甲氧西林（MRSA）。采用PCR扩增法检测以下基因；以及SCCmec类型。
结果
在收集的251份拭子中，31例（12.4%）被鉴定为携带者，其中14株（14/31；45.2%）分离株为MRSA。检测到的最常见基因是和，分别为22.6%和9.7%。最常见的SCCmec类型是SCCmec IV型（28.6%）。
结论
我们发现MRSA鼻携带率处于高水平，在被调查医院必须被视为一个重大的医疗保健问题。严格执行感染控制政策和合理使用抗生素是控制医院传播的主要支柱。
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