Kalambry Aimé Césaire, Potindji Tchamou Malraux Fleury, Guindo Ibrehima, Kassogue Ambara, Kambire Dinanibè, Dramé Boubacar Sidiki Ibrahim, Yéna Sadio, Doumbia Seydou, Diakité Mahamadou
Laboratory Teaching Hospital "Hôpital du Mali".
Ecole Supérieure des Techniques Biologiques et Alimentaires, Université de Lomé, Lomé, Togo.
Res Sq. 2024 May 23:rs.3.rs-3579825. doi: 10.21203/rs.3.rs-3579825/v1.
one of the pathogens strongly implicated in hospital infections. Data on the resistance and molecular characteristics of this bacterium are rare in Mali.
This study aimed to evaluate the antibiotic resistance patterns, virulence factors of isolates from pleural fluid infections in hospitalized patients.
Pleural effusion samples were obtained by thoracentesis for bacteriological examination from October 2021 to December 2022 at the "Hôpital du Mali" teaching hospital. Comorbidities such as HIV/AIDS and diabetes were assessed. Standard microbiological procedures were used for bacterial identification. The disk diffusion method was used to identify methicillin-resistant . The PCR amplification method was used to detect the following genes: , , , , and .
This study analyzed 6096 samples from inpatients and found a pooled frequency of bacterial pleuritis of 526 (8.6%) in thoracic surgery and pediatric wards. was isolated in 52 (9.88%) cases, of which 39 (75%) isolates were MRSA. There was no significant difference between the sexes (). The median age of the patients was 30 years. All isolates showed resistance to penicillin-G. The leucocidin toxin was detected in 7.7% of thoracic surgery patients, but , , , and toxins were not found.
In this study, we found a high frequency of (and MRSA) in pleurisy patients at the "Hôpital du Mali". Only the leukocidin was found. The empirical treatment protocol for pleurisy may need revision. Clindamycin, linezolid, teicoplanin, daptomycin, fosfomycin, vancomycin, moxifloxacin and fusidic acid were the most active antibiotics on our isolates in this study. Infection prevention measures, active surveillance, and effective therapeutic options are recommended.
是医院感染中密切相关的病原体之一。在马里，关于这种细菌的耐药性和分子特征的数据很少。
本研究旨在评估住院患者胸腔积液感染分离株的抗生素耐药模式和毒力因子。
2021年10月至2022年12月，在“马里医院”教学医院通过胸腔穿刺术获取胸腔积液样本进行细菌学检查。评估了如艾滋病毒/艾滋病和糖尿病等合并症。采用标准微生物学程序进行细菌鉴定。采用纸片扩散法鉴定耐甲氧西林菌。采用聚合酶链反应扩增法检测以下基因：、、、、和。
本研究分析了6096份住院患者样本，发现在胸外科和儿科病房细菌性胸膜炎的合并频率为526例（8.6%）。在52例（9.88%）病例中分离出，其中39例（75%）分离株为耐甲氧西林金黄色葡萄球菌。性别之间无显著差异（）。患者的中位年龄为30岁。所有分离株均对青霉素G耐药。在7.7%的胸外科患者中检测到杀白细胞素毒素，但未发现、、和毒素。
在本研究中，我们发现“马里医院”胸膜炎患者中（和耐甲氧西林金黄色葡萄球菌）的频率很高。仅发现了杀白细胞素。胸膜炎的经验性治疗方案可能需要修订。在本研究中，克林霉素、利奈唑胺、替考拉宁、达托霉素、磷霉素、万古霉素、莫西沙星和夫西地酸是对我们的分离株最有效的抗生素。建议采取感染预防措施、主动监测和有效的治疗选择。
