Jiang Shiyu, Li Li, Li Limin, Zheng Xueling, Li Zhengzhe, Song Xin
College of Grain and Food Science Henan University of Technology Zhengzhou China.
Food Sci Nutr. 2020 Feb 6;8(3):1345-1354. doi: 10.1002/fsn3.1366. eCollection 2020 Mar.
Being classified within the , has been proved to degrade lignocellulose, a major component of wheat bran fiber. During the fermentation of lignocellulose by strain, a large number of intermediate products are produced, which affect the further degradation of lignocellulose. Therefore, it is essential to analyze the fermentation intermediates for study on the degradation mechanism of wheat bran fiber. In this study, the effectiveness of fermentation of wheat bran fiber by strain was confirmed via scanning electron microscopy. Additionally, the results of gas chromatography-mass spectrometry indicated that the strain could degrade wheat bran fiber and produce several aromatic compounds, and that the number of products obtained after 7 days of fermentation was significantly lower than that after 3 days of fermentation. It has also been demonstrated that diisooctyl phthalate and 9-octadecenamide belong to metabolites produced during the fermentation of wheat bran fiber, by culturing with wheat bran fiber and glucose as carbon source, respectively. Moreover, by conducting an ultraviolet wavelength scanning of the culture liquid containing vanillin fermented by , it has been indicated that the strain could degrade vanillin and further demonstrated that the strain has the ability to degrade wheat bran fiber. Furthermore, adding the products of wheat bran fiber fermented for 3 days by could improve the elasticity of the dough sheet.
被归类于 ,已被证明可降解木质纤维素,这是麦麸纤维的主要成分。在 菌株对木质纤维素的发酵过程中,会产生大量中间产物,这些中间产物会影响木质纤维素的进一步降解。因此,分析发酵中间产物对于研究麦麸纤维的降解机制至关重要。在本研究中,通过扫描电子显微镜证实了 菌株对麦麸纤维发酵的有效性。此外,气相色谱 - 质谱分析结果表明,该菌株可降解麦麸纤维并产生几种芳香族化合物,且发酵7天后获得的产物数量明显低于发酵3天后的数量。通过分别以麦麸纤维和葡萄糖作为碳源培养 ,还证明了邻苯二甲酸二异辛酯和9 - 十八碳烯酰胺属于麦麸纤维发酵过程中产生的代谢产物。此外,通过对含有由 发酵的香草醛的培养液进行紫外波长扫描,表明该菌株可降解香草醛,并进一步证明该菌株具有降解麦麸纤维的能力。此外,添加由 发酵3天的麦麸纤维产物可提高面片的弹性。