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[肌红蛋白不同测定方法的信息价值发展及比较:固相免疫酶测定法和血凝试验]

[Development and comparisons of information value of different methods of myoglobin determination: solid phase immunoenzyme assay and hemagglutination test].

作者信息

Rott G M, Lapshina G M, Rozhinskaia I V, Letova O A, Blinova T V

出版信息

Vopr Med Khim. 1988 Sep-Oct;34(5):124-9.

PMID:3218127
Abstract

Optimal conditions were developed for estimation of myoglobin using immunoenzyme assay (ELISA) of competitive and "sandwich" types. Informative efficiency of the ELISA procedures was compared with commercially available hemagglutination test "Erythrocyte diagnosticum for myoglobin detection; dry immunoglobulin". Efficiency of these assays both ELISA and hemagglutination test was found to be similar although hemagglutination is a semiquantitative procedure. The commercially available preparation exhibited some advantages; it is rapid, simple and reproducible. The hemagglutination test may be used as an express analysis in urgent medicine as well as in laboratories which do not possess special biochemical equipment.

摘要

采用竞争性和“夹心”型免疫酶测定法(ELISA),确定了用于估计肌红蛋白的最佳条件。将ELISA方法的信息效率与市售的血凝试验“用于检测肌红蛋白的红细胞诊断试剂;干燥免疫球蛋白”进行了比较。尽管血凝试验是一种半定量方法,但发现这两种检测方法(ELISA和血凝试验)的效率相似。市售制剂具有一些优点;它快速、简单且可重复。血凝试验可用作急救医学中的快速分析方法,也适用于没有特殊生化设备的实验室。

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