Department of Anatomy, Faculty of Veterinary Medicine, University of Erciyes, Kayseri, Turkey.
Department of Anatomy, Faculty of Veterinary Medicine, University of On Dokuz Mayıs, Samsun, Turkey.
Anat Histol Embryol. 2020 Jul;49(4):478-485. doi: 10.1111/ahe.12551. Epub 2020 Mar 17.
The pecten oculi is a highly vascularized and pigmented organ that projects from the optic disc into the vitreous body in the avian eye. In this study, the pecten oculi of Turkey's native Gerze chicken was examined by light and scanning electron microscopy. Furthermore, the localization of some adherens junction components (E-cadherin and pan-cadherin) in intact vessels of the blood-retina barrier was investigated by immunohistochemistry. In the Gerze chicken, the pecten oculi was a thin structure, which was located over the head of the discus nervi optici and projected from the retina into the corpus vitreum. The pecten oculi consisted of 18-21 highly vascularized pleats, joined apically by a bridge and resembled an accordion in appearance. Hyalocytes and melanocytes were observed around the small and large vessels. The morphometric data of the pecten oculi showed that there were no statistical differences in terms of sex. The immunohistochemical analysis of the pecten oculi, which is used as a model for the investigation of the formation and maturation of the barrier properties in the central nervous system, revealed cytoplasmic E-cadherin and pan-cadherin immunoreactivity in the endothelial cells of the small, large and capillary vessels. These observations suggest that while the morphological and histological structure of the Gerze chicken's pecten oculi was generally similar to that of other diurnal domestic birds, the pecten oculi, a model system for vascular differentiation and the blood-retina barrier, expressed different cadherins.
眼部梳状突是一个高度血管化和色素沉着的器官，从视神经盘突出到禽类眼睛的玻璃体中。在这项研究中，通过光镜和扫描电子显微镜检查了土耳其本土 Gerze 鸡的眼部梳状突。此外，通过免疫组织化学研究了某些黏着连接成分（E-钙黏蛋白和pan-钙黏蛋白）在血视网膜屏障完整血管中的定位。在 Gerze 鸡中，眼部梳状突是一个薄的结构，位于视神经盘头部上方，从视网膜突入玻璃体。眼部梳状突由 18-21 个高度血管化的褶组成，顶部由桥连接，外观类似于手风琴。在小血管和大血管周围观察到了星状胶质细胞和成黑素细胞。眼部梳状突的形态学数据表明，其在性别方面没有统计学差异。眼部梳状突的免疫组织化学分析，作为研究中枢神经系统中屏障特性形成和成熟的模型，显示小血管、大血管和毛细血管内皮细胞中存在细胞质 E-钙黏蛋白和 pan-钙黏蛋白免疫反应性。这些观察结果表明，虽然 Gerze 鸡眼部梳状突的形态和组织学结构通常与其他昼行性家禽相似，但眼部梳状突作为血管分化和血视网膜屏障的模型系统，表达了不同的钙黏蛋白。
