Department of Orthopaedics, The 4th Affiliated Hospital of China Medical University, Shenyang, China.
DNA Cell Biol. 2020 May;39(5):747-755. doi: 10.1089/dna.2019.5002. Epub 2020 Mar 17.
Polo-like kinase 1 (PLK1) is a ubiquitous serine/threonine protein kinase. It is reported to be involved in the occurrence and progression of various human cancers. In the present study, we explored the role and molecular mechanism of in the proliferation of osteosarcoma (OS) cells. We found that expression was higher in MG63/Dox cells than in MG63 cells, while inhibiting or interfering with the level of suppressed cell proliferation of MG63/Dox cells. TargetScan analysis predicted that miR-4779 would interact with the 3'-UTR of PLK1 mRNAs and also inhibit cell autophagy of MG63/Dox cells. The data demonstrated that miR-4779 negatively regulates the expression of , and both miR-4779 and regulate cell proliferation and cell apoptosis of MG63/Dox cells, processes that are involved in the drug resistance of OS cells.
丝氨酸/苏氨酸蛋白激酶 1（PLK1）是一种普遍存在的蛋白激酶。有报道称其参与了多种人类癌症的发生和发展。在本研究中，我们探讨了在骨肉瘤（OS）细胞增殖中的作用和分子机制。我们发现，在 MG63/Dox 细胞中的表达高于在 MG63 细胞中的表达，而抑制或干扰的水平则抑制了 MG63/Dox 细胞的增殖。TargetScan 分析预测 miR-4779 会与 PLK1 mRNAs 的 3'-UTR 相互作用，并抑制 MG63/Dox 细胞的自噬。数据表明，miR-4779 负调控的表达，miR-4779 和均调节 MG63/Dox 细胞的增殖和细胞凋亡，这些过程涉及 OS 细胞的耐药性。
