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鉴定、分子特征分析及石蒜属中 JAZ 基因的表达

Identification, molecular characterization and expression of JAZ genes in Lycoris aurea.

机构信息

Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, China.

College of Life and Health Science, Anhui Science and Technology University, Fengyang, China.

出版信息

PLoS One. 2020 Mar 17;15(3):e0230177. doi: 10.1371/journal.pone.0230177. eCollection 2020.

Abstract

Jasmonates (JAs) are key phytohormones involved in regulation of plant growth and development, stress responses, and secondary metabolism. It has been reported that treatments with JAs could increase the contents of Amaryllidaceae alkaloids in Amaryllidaceae plants. Jasmonate ZIM (zinc-finger inflorescence meristem) domain (JAZ) proteins are key components in JA signal processes. However, JAZ proteins have not been characterized in genus Lycoris. In this study, we identified and cloned seven differentially expressed JAZ genes (namely LaJAZ1-LaJAZ7) from Lycoris aurea. Bioinformatic analyses revealed that these seven LaJAZ proteins contain the ZIM domain and JA-associated (Jas, also named CCT_2) motif. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis revealed that these LaJAZ genes display different expression patterns in L. aurea tissues, and most of them are inducible when treated with methyl jasmonate (MeJA) treatment. Subcellular localization assay demonstrated that LaJAZ proteins are localized in the cell nucleus or cytoplasm. In addition, LaJAZ proteins could interact with each other to form homodimer and/or heterodimer. The findings in this study may facilitate further functional research of the LaJAZ genes, especially the potential regulatory mechanism of plant secondary metabolites including Amaryllidaceae alkaloids in L. aurea.

摘要

茉莉酸(JAs)是参与植物生长发育、应激反应和次生代谢调节的关键植物激素。有报道称,JAs 的处理可以增加石蒜科植物中石蒜科生物碱的含量。茉莉酸 ZIM(锌指花序分生组织)结构域(JAZ)蛋白是 JA 信号过程中的关键组成部分。然而,JAZ 蛋白在石蒜属中尚未得到表征。在这项研究中,我们从忽地笑中鉴定和克隆了七个差异表达的 JAZ 基因(即 LaJAZ1-LaJAZ7)。生物信息学分析表明,这七个 LaJAZ 蛋白含有 ZIM 结构域和 JA 相关(Jas,也称为 CCT_2)基序。定量逆转录聚合酶链反应(qRT-PCR)分析表明,这些 LaJAZ 基因在忽地笑组织中表现出不同的表达模式,并且大多数基因在茉莉酸甲酯(MeJA)处理下是可诱导的。亚细胞定位实验表明,LaJAZ 蛋白定位于细胞核或细胞质中。此外,LaJAZ 蛋白可以相互作用形成同源二聚体和/或异源二聚体。本研究的结果可能有助于进一步研究 LaJAZ 基因的功能,特别是包括忽地笑中石蒜科生物碱在内的植物次生代谢物的潜在调控机制。

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