Lin Wenqian, Xing Sheng, Jin Yongcan, Lu Xiaomin, Huang Caoxing, Yong Qiang
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
Department of Paper Science and Technology, Nanjing Forestry University, Nanjing 210037, China.
Bioresour Technol. 2020 Jun;306:123163. doi: 10.1016/j.biortech.2020.123163. Epub 2020 Mar 10.
Deep eutectic solvent (DES) is a promising pretreatment for improving enzymatic digestibility of lignocellulosic material by altering the physicochemical properties. However, few work has been done to quantitatively analysis the physicochemical properties changes of lignocellulosic material with enzymatic digestibility. In this work, DES pretreatment with different molar ratios of choline chloride/lactic acid was carried out on bamboo residues and respective enzymatic digestibility was investigated and linearly fitted with corresponding physicochemical features changes of the pretreated bamboo residues. Results showed that enzymatic digestibility of DES-pretreated bamboo residues was enhanced with the increasing molar ratio of choline chloride/lactic acid, which was due to DES pretreatment's ability to remove lignin and xylan, reduce the degree of polymerization of cellulose, enhance the crystallite size of cellulose, and improve cellulose accessibility. Several compelling linear correlations (R = 0.6-0.9) were observable between enzymatic digestibility and these changes of physicochemical properties, demonstrating how DES pretreatment improve the enzymatic digestibility.
深共熔溶剂（DES）是一种很有前景的预处理方法，可通过改变木质纤维素材料的物理化学性质来提高其酶解消化率。然而，关于定量分析木质纤维素材料物理化学性质变化与酶解消化率之间关系的研究却很少。在本研究中，采用不同摩尔比的氯化胆碱/乳酸对竹渣进行DES预处理，并研究了各自的酶解消化率，同时将其与预处理竹渣相应的物理化学特征变化进行线性拟合。结果表明，随着氯化胆碱/乳酸摩尔比的增加，DES预处理竹渣的酶解消化率提高，这是由于DES预处理具有去除木质素和木聚糖、降低纤维素聚合度、增大纤维素微晶尺寸以及提高纤维素可及性的能力。在酶解消化率与这些物理化学性质变化之间可观察到几个显著的线性相关性（R = 0.6 - 0.9），这表明了DES预处理是如何提高酶解消化率的。
