处于怀孕和哺乳期的小鼠胰岛素分泌的长期增加。
Long-term increase of insulin secretion in mice subjected to pregnancy and lactation.
作者信息
Vicente Julia Modesto, Santos-Silva Junia Carolina, Teixeira Caio Jordão, de Souza Dailson Nogueira, Vettorazzi Jean Franciesco, Furtuoso Fabiola Sales, Adabo Isabel Gouveia, Sato Fabio Takeo, Vinolo Marco Aurélio Ramirez, Carneiro Everardo Magalhães, Bordin Silvana, Anhê Gabriel Forato
机构信息
Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, Campinas, Brazil.
Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, Campinas, Brazil.
出版信息
Endocr Connect. 2020 Apr;9(4):299-308. doi: 10.1530/EC-20-0020.
PURPOSE
Observational studies show that longer breastfeeding periods reduce maternal risk of type 2 diabetes mellitus. However, it is currently unknown if the long-term benefits of breastfeeding for maternal glucose homeostasis are linked to changes in the endocrine pancreas.
METHODS
We presently evaluated functional, morphological and molecular aspects of the endocrine pancreas of mice subjected to two sequential cycles of pregnancy and lactation (L21). Age-matched mice not allowed to breastfeed (L0) and virgin mice were used as controls.
RESULTS
L21 mice exhibited increased tolerance and increased glucose-stimulated insulin secretion (GSIS) by isolated islets. Pancreatic islets of L21 mice did not present evident morphological changes to justify the increased GSIS. On the other hand, islets of L21 mice exhibited a reduction in Cavb3 and Kir6.2 expression with concordant increased intracellular Ca2+ levels after challenge with glucose.
CONCLUSION
Altogether, the present findings show the breastfeeding exerts long-term benefits for maternal endocrine pancreas by increasing intracellular Ca2+ levels and GSIS.
目的
观察性研究表明，较长的母乳喂养期可降低母亲患2型糖尿病的风险。然而，目前尚不清楚母乳喂养对母亲葡萄糖稳态的长期益处是否与内分泌胰腺的变化有关。
方法
我们目前评估了经历两个连续妊娠和哺乳期（L21）的小鼠内分泌胰腺的功能、形态和分子方面。将未进行母乳喂养的年龄匹配小鼠（L0）和处女小鼠作为对照。
结果
L21小鼠表现出耐受性增加，分离的胰岛对葡萄糖刺激的胰岛素分泌（GSIS）增加。L21小鼠的胰岛未呈现明显的形态学变化来解释GSIS的增加。另一方面，L21小鼠的胰岛在用葡萄糖刺激后，Cavb3和Kir6.2表达降低，细胞内Ca2+水平相应升高。
结论
总之，目前的研究结果表明，母乳喂养通过提高细胞内Ca2+水平和GSIS对母亲的内分泌胰腺产生长期益处。
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