Department of General Surgery, Hunan Children's Hospital, Changsha, China.
Department of General Surgery, Hunan Children's Hospital, Changsha, China,
Pathobiology. 2020;87(3):161-170. doi: 10.1159/000505131. Epub 2020 Mar 17.
MiR-203 has been shown to participate in multiple malignancies, but the role of miR-203 in hepatoblastoma (HB) remains unclear. The aim of our study was to investigate the effects of miR-203 in HB.
A total of 15 pairs of HB tissues and para-tumour normal tissues were collected for the experiments. RT-qPCR and Western blotting were performed to detect the expression of CRNDE, miR-203, and VEGFA at the mRNA and/or protein levels, respectively. A dual luciferase assay verified the target relationship between miR-203 and the 3'UTR of VEGFA as well as miR-203 and CRNDE. In addition, MTT, wound healing, and tube formation assays were performed to assess the effects of miR-203, VEGFA, and CRNDE on cell proliferation, migration, and angiogenesis, respectively.
Our data revealed that miR-203 expression was decreased in HB tissues, while long non-coding RNA (lncRNA) CRNDE expression was increased. The dysregulation of miR-203 and CRNDE was closely related to tumour size and stage. Moreover, overexpression of miR-203 inhibited angiogenesis. A dual luciferase assay verified that VEGFA is a direct target of miR-203 and that CRNDE binds to miR-203. Furthermore, our results showed that miR-203 suppressed cell viability, migration, and angiogenesis by regulating VEGFA expression. Additionally, it was confirmed that CRNDE promoted angiogenesis by negatively regulating miR-203 expression.
lncRNA CRNDE targets the miR-203/VEGFA axis and promotes angiogenesis in HB. These results provide insight into the underlying mechanisms of HB and indicate that CRNDE and miR-203 might be potential targets for HB therapy.
已有研究表明 miR-203 参与多种恶性肿瘤，但 miR-203 在肝母细胞瘤（HB）中的作用尚不清楚。本研究旨在探讨 miR-203 在 HB 中的作用。
收集 15 对 HB 组织及其癌旁正常组织进行实验。采用 RT-qPCR 和 Western blot 分别检测 CRNDE、miR-203 和 VEGFA 的 mRNA 和/或蛋白水平表达。双荧光素酶报告实验验证 miR-203 与 VEGFA 3'UTR 以及 miR-203 与 CRNDE 的靶关系。此外，MTT、划痕愈合和管形成实验分别评估 miR-203、VEGFA 和 CRNDE 对细胞增殖、迁移和血管生成的影响。
研究数据显示，miR-203 在 HB 组织中表达降低，而长链非编码 RNA（lncRNA）CRNDE 表达升高。miR-203 和 CRNDE 的失调与肿瘤大小和分期密切相关。此外，过表达 miR-203 抑制血管生成。双荧光素酶报告实验验证 VEGFA 是 miR-203 的直接靶基因，CRNDE 与 miR-203 结合。此外，研究结果表明，miR-203 通过调节 VEGFA 表达抑制细胞活力、迁移和血管生成。此外，还证实 CRNDE 通过负调控 miR-203 表达促进血管生成。
lncRNA CRNDE 靶向 miR-203/VEGFA 轴并促进 HB 血管生成。这些结果为 HB 的潜在机制提供了深入的了解，并表明 CRNDE 和 miR-203 可能是 HB 治疗的潜在靶点。
