基于 Phenosafranin 的比色传感平台，通过格里厄斯测定法实现亚硝酸盐检测。
Phenosafranin-Based Colorimetric-Sensing Platform for Nitrite Detection Enabled by Griess Assay.
机构信息
Key Laboratory of Eco-Environment of Three Gorges Region of Ministry of Education, College of Environment and Ecology, Chongqing University, Chongqing 400045, China.
Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province, Yibin University, Yibin 644000, China.
出版信息
Sensors (Basel). 2020 Mar 9;20(5):1501. doi: 10.3390/s20051501.
A facile and effective colorimetric-sensing platform based on the diazotization of phenosafranin for the detection of NO 2 - under acidic conditions using the Griess assay is presented. Diazotization of commercial phenosafranin produces a color change from purplish to blue, which enables colorimetric quantitative detection of NO 2 - . Optimal detection conditions were obtained at a phenosafranin concentration of 0.25 mM, HCl concentration of 0.4 M, and reaction time of 20 min. Under the optimized detection conditions, an excellent linearity range from 0 to 20 μM was obtained with a detection limit of 0.22 μM. Favorable reproducibility and selectivity of the colorimetric sensing platform toward NO 2 - were also verified. In addition, testing spiked ham sausage, bacon, and sprouts samples demonstrated its excellent practicability. The presented colorimetric sensing platform is a promising candidate for the detection of NO 2 - in real applications.
提出了一种基于酚藏花红重氮化反应的比色传感平台，用于在酸性条件下使用格里斯试剂检测 NO 2 - 。商业酚藏花红的重氮化反应会导致颜色从紫红色变为蓝色，从而实现了对 NO 2 - 的比色定量检测。在酚藏花红浓度为 0.25 mM、HCl 浓度为 0.4 M、反应时间为 20 min 的最佳检测条件下，获得了优异的线性范围从 0 到 20 μM，检测限为 0.22 μM。该比色传感平台对 NO 2 - 还表现出了良好的重现性和选择性。此外，对加标火腿香肠、培根和豆芽样品的测试证明了其出色的实用性。所提出的比色传感平台有望成为实际应用中检测 NO 2 - 的候选方法。
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