Hooft P, van de Voorde H
Laboratory of Forensic Medicine, University of Leuven, Belgium.
Z Rechtsmed. 1988;101(1):41-4. doi: 10.1007/BF00205323.
Samples of complete human semen were incubated in gastric juice for different time periods at 37 degrees C, and by simulating the post-mortem temperature decrease of the human body. The changes in the spermatozoa were similar in both experiments. Short incubation specimens were examined directly with interference contrast microscopy and showed an almost immediate immobilization of spermatozoa when they were brought in contact with gastric juice. Specimens with longer incubation periods were stained with alcalic fuchsine and examined by immersion microscopy. There was a morphologically stable plateau for the heads of the spermatozoa for up to 6 h of incubation. The tails disappeared progressively in the first 45 min. After more than 6 h of incubation a progressive swelling and lysis of the heads was observed. Spermatozoa could be recognized for up to 7 days of incubation.
将完整的人类精液样本在37摄氏度下于胃液中孵育不同时间段，并通过模拟人体死后体温下降的情况。在两个实验中精子的变化相似。短孵育时间的样本直接用相差显微镜检查，结果显示精子与胃液接触后几乎立即失去活力。较长孵育时间的样本用碱性品红染色，然后用油浸显微镜检查。在长达6小时的孵育过程中，精子头部在形态上保持稳定。尾巴在最初的45分钟内逐渐消失。孵育超过6小时后，观察到头部逐渐肿胀并溶解。在长达7天的孵育过程中都可以识别出精子。
