哺乳动物细胞、小鼠和酵母中自噬性线粒体的检测。

Detection of mitophagy in mammalian cells, mice, and yeast.

作者信息

Calvelli Hannah, Krigman Judith, Onishi Mashun, Narendra Derek P, Sun Nuo, Okamoto Koji

机构信息

Inherited Movement Disorders Unit, Neurogenetics Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, United States.

Departments of Physiology and Cell Biology, The Ohio State University Wexner Medical Center, Columbus, OH, United States; Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH, United States.

出版信息

Methods Cell Biol. 2020;155:557-579. doi: 10.1016/bs.mcb.2019.10.006. Epub 2019 Nov 27.

DOI:10.1016/bs.mcb.2019.10.006

PMID:32183977

Abstract

Selective elimination of superfluous or dysfunctional mitochondria is a fundamental process conserved among both uni- and multicellular eukaryotes, contributing to mitochondrial quality and quantity control. This process depends on autophagy, a cellular self-eating membrane trafficking system, and is thus called mitophagy. In this chapter, we describe methods to detect mitophagy in mammalian cells, mice, and yeast.

摘要

选择性清除多余或功能失调的线粒体是单细胞和多细胞真核生物中保守的基本过程，有助于线粒体质量和数量的控制。这个过程依赖于自噬，一种细胞自噬膜运输系统，因此被称为线粒体自噬。在本章中，我们描述了在哺乳动物细胞、小鼠和酵母中检测线粒体自噬的方法。