Department of Gastroenterology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, People's Republic of China; Department of Gastroenterology, Cangzhou Central Hospital, Cangzhou, Hebei 061001, People's Republic of China.
Department of Gastroenterology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, People's Republic of China.
Acta Histochem. 2020 May;122(4):151538. doi: 10.1016/j.acthis.2020.151538. Epub 2020 Mar 14.
Esophageal cancer is a digestive tract malignancy, ranking sixth among the world's deadliest tumor incidence. However, the pathogenesis of esophageal cancer is complex and the prognosis remains poor. Therefore, in-depth study of the pathogenesis and developing effective treatments are of great value for esophageal cancer. β-elemene is a natural monomeric compound derived from the Chinese herbal Curcuma wenyujin. β-elemene has been reported to have anti-tumor effects and used as an adjunct to clinical therapy for multiple cancers. This study aims to explore the effects of β-elemene on esophageal cancer and its related molecular mechanisms.
TE-1 and KYSE-150 cells were used to evaluate the activity of β-elemene on esophageal cancerin vitro and in vivo. Western blot was performed for protein expression assessment. CCK8 assay and cell cycle analysis were used for proliferation testing. Flow cytometry was performed for apoptosis detection. Wound healing assay was subjected to assess the migration ability. Transwell chamber assay was applied to assess the invasion ability. HE staining, TUNEL staining and immunohistochemical staining were used to evaluate the changes in tumor tissues.
We found that β-elemene treatment suppressed proliferation, as well as induced apoptosis of esophageal cancer cells. In addition, β-elemene inhibited the migration and invasion ability of esophageal cancer cells. Furthermore, β-elemene exerted its effects against esophageal cancer by specifically regulating AKT signaling, thereby controlling the expression of PD-L1.
β-elemene inhibits proliferation and metastasis of esophageal cancer cells by regulating the phosphorylation of AKT.
食管癌是一种消化道恶性肿瘤，在全球肿瘤致死率中排名第六。然而，食管癌的发病机制复杂，预后仍然较差。因此，深入研究其发病机制并开发有效的治疗方法对食管癌具有重要意义。β-榄香烯是一种从莪术中药材中提取的天然单一组分化合物。已有报道称β-榄香烯具有抗肿瘤作用，并被用作多种癌症的临床辅助治疗药物。本研究旨在探讨β-榄香烯对食管癌的作用及其相关分子机制。
采用 TE-1 和 KYSE-150 细胞在体外和体内评估β-榄香烯对食管癌的活性。通过 Western blot 检测蛋白表达水平。CCK8 检测和细胞周期分析用于增殖检测。流式细胞术用于检测细胞凋亡。划痕愈合实验用于评估迁移能力。Transwell 小室实验用于评估侵袭能力。HE 染色、TUNEL 染色和免疫组织化学染色用于评估肿瘤组织的变化。
我们发现β-榄香烯处理抑制了食管癌细胞的增殖，并诱导其凋亡。此外，β-榄香烯抑制了食管癌细胞的迁移和侵袭能力。进一步研究发现，β-榄香烯通过特异性调节 AKT 信号通路来发挥其对食管癌的作用，从而控制 PD-L1 的表达。
β-榄香烯通过调节 AKT 的磷酸化来抑制食管癌细胞的增殖和转移。
