DGIMI, Univ Montpellier, INRAE, Montpellier, France.
DGIMI, Univ Montpellier, INRAE, Montpellier, France.
Dev Comp Immunol. 2020 Jul;108:103676. doi: 10.1016/j.dci.2020.103676. Epub 2020 Mar 14.
The Steinernema carpocapsae-Xenorhabdus nematophila association is a nematobacterial complex used in biological control of insect crop pests. The infection success of this dual pathogen strongly depends on its interactions with the host's immune system. Here, we used the lepidopteran pest Spodoptera frugiperda to analyze the respective impact of each partner in the induction of its immune responses. First, we used previously obtained RNAseq data to construct the immunome of S. frugiperda and analyze its induction. We then selected representative genes to study by RT-qPCR their induction kinetics and specificity after independent injections of each partner. We showed that both X. nematophila and S. carpocapsae participate in the induction of stable immune responses to the complex. While X. nematophila mainly induces genes classically involved in antibacterial responses, S. carpocapsae induces lectins and genes involved in melanization and encapsulation. We discuss putative relationships between these differential inductions and the pathogen immunosuppressive strategies.
斯氏线虫-共生菌复合体是一种用于防治鳞翅目农业害虫的生物防治制剂，其中包含斯氏线虫和共生菌 Xenorhabdus nematophila。该双病原体复合体的感染成功与否强烈依赖于其与宿主免疫系统的相互作用。在这里，我们利用鳞翅目害虫玉米螟 Spodoptera frugiperda 来分析每个伙伴在诱导其免疫反应方面的各自作用。首先，我们使用先前获得的 RNAseq 数据构建了 S. frugiperda 的免疫组，并分析了其诱导情况。然后，我们选择了代表性基因，通过 RT-qPCR 研究了在单独注射每个伙伴后它们的诱导动力学和特异性。结果表明，X. nematophila 和 S. carpocapsae 均参与了对复合体的稳定免疫反应的诱导。虽然 X. nematophila 主要诱导经典的参与抗细菌反应的基因，但 S. carpocapsae 诱导凝集素和参与黑化和包被的基因。我们讨论了这些差异诱导与病原体免疫抑制策略之间的可能关系。
