Liu Xian, Wang Yuzhe, Liu Yuhui, Chen Hui, Hu Yalin
Forest Ecology and Stable Isotope Research Center, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, China.
Xinkou Forest Farm, Fujian Agriculture and Forestry University, Sanming, China.
Front Microbiol. 2020 Feb 28;11:181. doi: 10.3389/fmicb.2020.00181. eCollection 2020.
Successive rotation and monoculture, as common silvicultural practices, are extensively applied worldwide, particularly in subtropical Chinese fir () plantations in southern China. Although regeneration failure and productivity decline are frequently observed in continuous monoculture plantations, the potential mechanisms are still unclear. In this study, high-throughput sequencing was used to compare the diversity and composition of bacterial and fungal communities among different generations of Chinese fir plantation (first rotation, FRP; second rotation, SRP; third rotation, TRP) and natural forest (NF) in December and June. Our results showed significant declines in richness and diversity of bacterial and fungal communities in TRP compared with FRP and SRP, but no significant difference between FRP and SRP. The fungal phyla with high relative abundance were (12.9-76.9%) and (14.3-52.8%), while the bacterial phyla with high relative abundance were (39.1-57.7%) and (21.2-39.5%) in all treatments at both sampling months. On average, the relative abundance of in TRP increased by 53.4%, while that of decreased by 37.1% compared with FRP and SRP. Moreover, soil NH -N, pH, and DOC appear to be the key factors in shaping the fungal communities, while soil NH -N, DOCN, and AP primarily drive the changes in bacterial communities. Collectively, our findings highlighted the alteration of soil bacterial and fungal communities induced by changes in soil nutrient environment in different generations of continuously cultivated Chinese fir plantation.
连续轮伐和单一栽培作为常见的造林实践，在全球范围内广泛应用，尤其是在中国南方亚热带杉木人工林中。尽管在连续单一栽培的人工林中经常观察到更新失败和生产力下降的情况，但其潜在机制仍不清楚。在本研究中，利用高通量测序技术，比较了12月和6月不同代杉木人工林（第一代轮伐期，FRP；第二代轮伐期，SRP；第三代轮伐期，TRP）和天然林（NF）中细菌和真菌群落的多样性和组成。我们的结果表明，与FRP和SRP相比，TRP中细菌和真菌群落的丰富度和多样性显著下降，但FRP和SRP之间没有显著差异。在两个采样月份的所有处理中，相对丰度较高的真菌门是担子菌门（12.9 - 76.9%）和子囊菌门（14.3 - 52.8%），而相对丰度较高的细菌门是变形菌门（39.1 - 57.7%）和酸杆菌门（21.2 - 39.5%）。平均而言,与FRP和SRP相比，TRP中镰刀菌属的相对丰度增加了53.4%，而木霉属的相对丰度下降了37.1%。此外，土壤铵态氮、pH值和溶解性有机碳似乎是塑造真菌群落的关键因素，而土壤铵态氮、溶解性有机碳氮和有效磷主要驱动细菌群落的变化。总的来说，我们的研究结果突出了不同代连续栽培杉木人工林土壤养分环境变化引起的土壤细菌和真菌群落的改变。
