Consun Chinese Medicines Research Centre for Renal Diseases, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong 999077, China.
HKBU Shenzhen Research Institute and Continuing Education, Shenzhen 518001, China.
J Tradit Chin Med. 2019 Jun;39(3):339-345.
To investigate the underlying mechanism of Bailian (Radix Ampelopsis Japonicae, BL) extract action on colorectal cancer (CRC).
We explored the involvement of β-catenin signaling on the anti-CRC effects of an BL ethanolic extract (BLE) in cell models by using the 3-(4,5-dimethylthiazol-2-yl)-2,5- iphenyltetrazolium bromide assay, immunofluorescent staining, luciferase assay, Western blot analysis and real-time quantitative polymerase chain reaction analysis. Anti-CRC compounds were quantified by high performance liquid chromatography.
The contents of gallic acid, catechin, and epicatechin in the BLE were 0.23, 1.25, and 0.18 g/kg, respectively. BLE-mediated cytotoxic and apoptotic effects were accompanied by lowered β-catenin/Tcf transcriptional activity, reduced β-catenin nuclear localization, and downregulated protein and mRNA levels of both β-catenin and molecules regulated by β-catenin.
The mechanism underpinning the anti-CRC effects of BLE may involve inhibition of β-catenin signaling. Further studies are necessary to establish the role of β-catenin signaling in the action of BLE-mediated anti-CRC effects.
探究白蔹提取物对结直肠癌（CRC）作用的潜在机制。
我们通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐法、免疫荧光染色法、荧光素酶检测、Western blot 分析和实时定量聚合酶链反应分析，探讨了 β-连环蛋白信号通路在白蔹乙醇提取物（BLE）抗 CRC 作用中的参与情况。采用高效液相色谱法对抗 CRC 化合物进行定量分析。
BLE 中没食子酸、儿茶素和表儿茶素的含量分别为 0.23、1.25 和 0.18 g/kg。BLE 介导的细胞毒性和凋亡作用伴随着β-连环蛋白/Tcf 转录活性降低、β-连环蛋白核定位减少以及β-连环蛋白及其受其调控的分子的蛋白和 mRNA 水平下调。
BLE 抗 CRC 作用的潜在机制可能涉及抑制 β-连环蛋白信号通路。需要进一步的研究来确定β-连环蛋白信号通路在 BLE 介导的抗 CRC 作用中的作用。
