隔药灸对克罗恩病大鼠结肠及中枢多巴胺水平和多巴胺受体 1 表达的影响。
Effect of herb-partitioned moxibustion on dopamine levels and dopamine receptor 1 expression in the colon and central nervous system in rats with Crohn's disease.
机构信息
Department of Mechanics and Engineering Science, Fudan University, Shanghai 200433, China.
Shanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, China.
出版信息
J Tradit Chin Med. 2019 Jun;39(3):356-363.
OBJECTIVE
To observe the effect of herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints in rats with Crohn's disease, and explore the underlying mechanism from dopamine (DA) and dopamine receptor 1 (D1R) in the colon, spinal dorsal horn and hypothalamus.
METHODS
The rats were randomly divided into the normal, model (CD), herb-partitioned moxibustion (Mox) and mesalazine (Mesa) groups. Damage in the colons was scored and observed by hematoxylin and eosin staining. DA and D1R protein expression in the colonic mucosa were detected by immunohistochemistry. The concentrations of DA and D1R in the spinal dorsal horn and hypothalamus were measured by enzyme-linked immunosorbent assay, and D1R mRNA expression was evaluated by quantitative real-time polymerase chain reaction.
RESULTS
In the colon, compared with the normal group, DA, D1R protein expressions and D1R mRNA expression were significantly higher in the model group, while decreased in the Mox group and the Mesa group. In the spinal dorsal horn and hypothalamus, compared with the normal group, the concentrations of DA and D1R, and the D1R mRNA expressions were significantly higher in the model group, and decreased in the Mox group and the Mesa group.
CONCLUSION
Herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints relieved ulceration in CD rats, the underlying mechanism maybe relative with the regulation of DA and D1R in the colon, spinal dorsal horn and hypothalamus by moxibustion.
目的
观察天枢（ST25）和气海（CV6）穴隔药灸对克罗恩病大鼠的作用，并从结肠、脊髓背角和下丘脑的多巴胺（DA）和多巴胺受体 1（D1R）探讨其作用机制。
方法
将大鼠随机分为正常组、模型组、隔药灸组和美沙拉嗪组。通过苏木精-伊红（HE）染色观察结肠损伤评分，免疫组织化学法检测结肠黏膜中 DA 和 D1R 蛋白的表达，酶联免疫吸附试验检测脊髓背角和下丘脑 DA 和 D1R 的浓度，实时定量聚合酶链反应检测 D1R mRNA 的表达。
结果
与正常组相比，模型组大鼠结肠中 DA、D1R 蛋白表达和 D1R mRNA 表达均显著升高，而隔药灸组和美沙拉嗪组则降低；与正常组相比，模型组大鼠脊髓背角和下丘脑 DA 和 D1R 浓度以及 D1R mRNA 表达均显著升高，而隔药灸组和美沙拉嗪组则降低。
结论
天枢和气海穴隔药灸可缓解 CD 大鼠的溃疡，其作用机制可能与艾灸对结肠、脊髓背角和下丘脑 DA 和 D1R 的调节有关。
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