School of Korean Medicine and Korean Medical Research Center for Healthy Aging, Pusan National University, Yangsan 50612, Republic of Korea.
Graduate Training Program of Korean Medicine for Healthy-aging, Pusan National University, Yangsan 50612, Republic of Korea.
J Tradit Chin Med. 2019 Feb;39(1):15-25.
To find out the combination of the extracts from Paeonia lactiflora Pallas (PL), Reh- mannia Glutinosa var. Purpurea Makino (RG), Perilla Frutescens var. Acuta Kudo (PF) to increase endometrial receptivity.
Herbal medicines were extracted with boiling water and polysaccharides were removed. We examined the effect of PL, RG, and PF (PRP), a most effective herbal formula deduced from constitutive ingredient herbs of Antai Yin which is composed of PRP, on the leukemia inhibitory factor (LIF) expression and endometrial receptivity.
The combination of the extracts from PRP induced the LIF expression in Ishikawa cells and increased the adhesion between Ishikawa and JAr cells. In addition, PRP-induced attachment of JAr cells onto Ishikawa cells and expression of adhesion molecules, ITGAV, ITGB5, CD44s, and L-selectin, are significantly reduced by knock-down of LIF expression.
Induced by the combination of the PRP extracts, the adhesion between trophoblast and endometrial cells are mediated by expression of LIF and adhesion molecules. Thus, we suggest the combination of the PRP extracts may be a novel therapy for enhancing embryo implantation rate.
探讨芍药（PL）、地黄（RG）、紫苏（PF）提取物的组合对增加子宫内膜容受性的作用。
采用水煮法提取中草药，去除多糖。我们研究了最有效的中草药配方 PRP（由 Antai Yin 的组成成分芍药、地黄、紫苏组成）对白血病抑制因子（LIF）表达和子宫内膜容受性的影响。
PRP 提取物的组合诱导 Ishikawa 细胞中 LIF 的表达，并增加了 Ishikawa 和 JAr 细胞之间的黏附。此外，PRP 诱导的 JAr 细胞附着到 Ishikawa 细胞上以及黏附分子 ITGAV、ITGB5、CD44s 和 L-选择素的表达，可被 LIF 表达的敲低显著减少。
PRP 提取物的组合诱导滋养细胞和子宫内膜细胞之间的黏附，这是通过 LIF 和黏附分子的表达介导的。因此，我们认为 PRP 提取物的组合可能是一种增强胚胎着床率的新疗法。
