口服黄芩乙醇提取物的大鼠潜在慢性肝毒性。

Potential chronic liver toxicity in rats orally administered an ethanol extract of Huangqin (Radix Scutellariae Baicalensis).

作者信息

Yi Yan, Zhao Yong, Li Chunying, Zhang Yushi, Bin Yang, Yuan Yalan, Pan Chen, Wang Lianmei, Liang Aihua

机构信息

Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.

出版信息

J Tradit Chin Med. 2018 Apr;38(2):242-256.

PMID:32186063

Abstract

OBJECTIVE

To investigate on the cytotoxicity and penetration enhancement effect of essential oils (EOs) from warming the interior medicinals (WIM) from Traditional Chinese Medicine (TCM).

METHODS

EOs were extracted from WIM of Bichengqie (Litseae Fructus), Dingxiang (Flos Syzygii Aromatici), Huajiao (Pericarpium Zanthoxyli Bungeani), and Xiaohuixiang (Fructus Foeniculi) with warm nature, and Ganjiang (Rhizoma Zingiberis), Gaoliangjiang (Rhizoma Alpiniae Officinari), Rougui (Cortex Cinnamomi Cassiae), and Wuzhuyu (Fructus Evodiae Rutaecarpae) with hot nature, respectively. Their chemical compositions were analyzed by gas chromatography-mass spectrometry (GC-MS). The cytotoxicity of the extracted eight EOs on HaCaT cells was measured and compared. Moreover, analyses of cell cycle and cell apoptosis were performed to investigate the cytotoxic mechanism. The transdermal penetration enhancement effects of the extracted eight EOs on ibuprofen were further compared by the modified Franz diffusion cell method.

RESULTS

The most abundant constituents in the extracted eight EOs were determined to be monoterpenes, especially oxygen-containing monoterpenes. The HaCaT cell cytotoxicity of EOs from WIM with hot nature were significantly (P = 0.020) higher than that with warm nature. Both ginger oil and zanthoxylum oil significantly induced G0/G1 phase arrestment in HaCaT cell cycle. For ginger oil from WIM with hot nature and zanthoxylum oil from WIM with warm nature, the main mechanisms of the cytotoxicity were found to be the induction of cellular necrosis and the cellular apoptosis, respectively. Furthermore, most of the tested EOs showed remarkable penetration enhancement activity on ibuprofen. However, no statistical significance (P = 0.18) was found between penetration enhancement activity of EOs from WIM with warm nature and EOs from WIM with hot nature.

CONCLUSION

With the enhanced penetration activity, the extracted EOs from the WIM demonstrated their significant effect of the cytotoxicity on the skin cells.

摘要

目的

研究中药温里药挥发油的细胞毒性及促渗透作用。

方法

分别从性温的荜澄茄（山鸡椒果实）、丁香（丁香花）、花椒（花椒果皮）、小茴香（小茴香果实）以及性热的干姜（干姜根茎）、高良姜（高良姜根茎）、肉桂（肉桂树皮）、吴茱萸（吴茱萸果实）中提取挥发油。采用气相色谱 - 质谱联用（GC-MS）分析其化学成分。测定并比较所提取的8种挥发油对HaCaT细胞的细胞毒性。此外，进行细胞周期和细胞凋亡分析以探究细胞毒性机制。采用改良的Franz扩散池法进一步比较所提取的8种挥发油对布洛芬的透皮促渗作用。

结果

所提取的8种挥发油中含量最丰富的成分是单萜类，尤其是含氧单萜。性热的温里药挥发油对HaCaT细胞的细胞毒性显著（P = 0.020）高于性温的温里药挥发油。姜油和花椒油均显著诱导HaCaT细胞周期阻滞于G0/G1期。对于性热的温里药干姜挥发油和性温的温里药花椒挥发油，发现其细胞毒性的主要机制分别是诱导细胞坏死和细胞凋亡。此外，大多数受试挥发油对布洛芬显示出显著的促渗透活性。然而，性温的温里药挥发油和性热的温里药挥发油的促渗透活性之间未发现统计学差异（P = 0.18）。