Zhang Shuhui, Xu Jia, Wang Chao, Feng Daofu, Dai Liangti, Kuang Xiangyu, Zhu Shuyi, Fang Yongchun, Chen Dongyan, He Xianghui, Feng Xizeng
State Key Laboratory of Medicinal Chemical Biology, The Key Laboratory of Bioactive Materials, Ministry of Education.
College of Life Science, Nankai University, Tianjin 300071, China.
J Tradit Chin Med. 2019 Dec;39(6):875-884.
To evaluate the effects of Rongchang capsule and Xifeng capsule on pentylenetetrazole- induced epilepsy in zebrafish larvae and to explore the possible mechanisms behind their actions.
We utilized a trajectory tracking system to monitor seizures in zebrafish larva to confirm that certain concentrations of Rongchang capsule and Xifeng capsule produce antiepileptic effects. c-fos expression was assessed by quantitative reverse transcription-polymerase chain reaction to validate the efficacy of the capsules. Rest/wake behavior and correlation analysis predicted the targets of Rongchang capsule and Xifeng capsule.
Larval movement times and total distances traveled by zebrafish larvae experiencing pentylenetetrazole (PTZ)-induced seizures were decreased by valproate treatment. Rongchang (500 μg/mL) and Xifeng (200 μg/mL) rescued the epileptic behaviors and down-regulated c-fos expression in the brains of larvae, which indicated antiepileptic effects. The rest/wake behavioral profiles showed that Rongchang and Xifeng differentially decreased rest time at night and increased larval locomotor activities during the day. Based on correlation between the actions of the two capsules and known compounds, we predicted that they might change rest/wake behaviors by affecting serotonin, GABAergic and histamine signaling pathways.
The efficacy of Rongchang capsule and Xifeng capsule in alleviating epilepsy-like behaviors and molecular responses was confirmed. Our study provides insight into the capsules' effect on epilepsy.
评估荣昌胶囊和熄风胶囊对戊四氮诱导的斑马鱼幼体癫痫的影响，并探讨其作用背后的可能机制。
我们利用轨迹跟踪系统监测斑马鱼幼体的癫痫发作，以确认一定浓度的荣昌胶囊和熄风胶囊具有抗癫痫作用。通过定量逆转录聚合酶链反应评估c-fos表达，以验证胶囊的疗效。静息/觉醒行为及相关性分析预测了荣昌胶囊和熄风胶囊的作用靶点。
丙戊酸治疗可减少经历戊四氮（PTZ）诱导癫痫发作的斑马鱼幼体的运动时间和总游动距离。荣昌（500μg/mL）和熄风（200μg/mL）可挽救癫痫行为，并下调幼体大脑中的c-fos表达，这表明其具有抗癫痫作用。静息/觉醒行为特征表明，荣昌和熄风可不同程度地减少夜间休息时间，并增加幼体白天的运动活动。基于两种胶囊与已知化合物作用之间的相关性，我们预测它们可能通过影响5-羟色胺、γ-氨基丁酸能和组胺信号通路来改变静息/觉醒行为。
证实了荣昌胶囊和熄风胶囊在减轻癫痫样行为和分子反应方面的疗效。我们的研究为胶囊对癫痫的作用提供了见解。
