Amity Institute of Biotechnology, Amity University, Noida, Sector 125, 201313, India.
Priyamvada Birla Aravind Eye Hospital, Kolkata, 700017, WB, India.
Infect Disord Drug Targets. 2021;21(2):284-288. doi: 10.2174/1871526520666200318111957.
Nowadays, the co-infection of different classes of pathogens is a major concern. The objective of this study was to develop a successful therapy for keratitis caused by the co-infection of Candida sp. with Pseudomonas sp, which is difficult to cure. The study is based on a 47 years old male farmer showing redness and watering in the right eye for 15-days. ; Methods: The microbiological examination was performed to isolate the causative organisms, i.e. Pseudomonas aeruginosa and Candida albicans. They were cultured separately along with their co-culture and treated with ciprofloxacin and amphotericin B during the growing stage to predict a definite cure. ; Results: Scanning electron microscope (SEM) results confirmed the inter-specific interaction between the two different types of microorganisms. Amphotericin-B and Ciprofloxacin showed the least MIC value for both organisms in co-culture. ; Conclusion: Treatment with Amphotericin-B and 5% ciprofloxacin effectively hindered the growth of Pseudomonas aeruginosa and Candida albicans, the co-infection of which caused keratitis. This therapy may be successfully implied for such cases of co-infection in the future.
如今，不同类病原体的混合感染是一个主要关注点。本研究旨在为假单胞菌和念珠菌混合感染引起的角膜炎寻找一种有效的治疗方法，因为这种感染很难治愈。本研究基于一名 47 岁男性农民的病例，他右眼出现红肿和流泪症状已经 15 天。方法：通过微生物学检查分离出致病生物体，即铜绿假单胞菌和白色念珠菌。将它们分别进行培养，并在生长阶段与共培养物一起用环丙沙星和两性霉素 B 进行治疗，以预测明确的治愈效果。结果：扫描电子显微镜（SEM）结果证实了两种不同类型微生物之间的种间相互作用。两性霉素 B 和环丙沙星在共培养物中对两种生物体的 MIC 值最低。结论：用两性霉素 B 和 5%环丙沙星治疗可以有效抑制铜绿假单胞菌和白色念珠菌的生长，这两种病原体的混合感染会导致角膜炎。这种治疗方法可能会在未来成功应用于此类混合感染病例。
