Zhao Lei, He Xingming, Todoh Masahiro
Division of Human Mechanical Systems and Design, Faculty of Engineering, Hokkaido University, Japan.
Division of Human Mechanical Systems and Design, Faculty of Engineering, Hokkaido University, Japan.
J Biomech. 2020 May 7;104:109738. doi: 10.1016/j.jbiomech.2020.109738. Epub 2020 Mar 3.
Biomimetic mineralization is a promising technique in biomedical applications. To understand the mechanical behavior of biomimetically mineralized collagen material (BMC), we examined the composition and structure of the mineral deposition in BMCs mineralized by the polymer-induced liquid precursor (PILP) process and applied wide angle x-ray scattering (WAXS) with in situ tensile testing to investigate the mineral-to-tissue co-deformation in the material. We found that the PILP process is able to achieve good biomimetic mineralization in bulk collagen matrix. Compositionally, the mineral deposition showed high crystallinity with no carbonation. However, the morphology of extrafibrillar mineral deposition and the preferential crystal orientation were different from natural bone. Further, the Young's modulus and mineral-to-tissue co-deformation ratio of the BMC were significantly lower than both natural bone and partially demineralized bone with similar mineral volume fraction. It was concluded that while biomimetic mineralization can achieve good mineral deposition volume in the BMC, the mechanical behavior of the material was different from natural bone.
仿生矿化是生物医学应用中一项很有前景的技术。为了解仿生矿化胶原材料（BMC）的力学行为，我们研究了通过聚合物诱导液相前驱体（PILP）工艺矿化的BMC中矿物质沉积的组成和结构，并应用广角X射线散射（WAXS）结合原位拉伸试验来研究材料中矿物质与组织的协同变形。我们发现，PILP工艺能够在块状胶原基质中实现良好的仿生矿化。在组成上，矿物质沉积显示出高结晶度且无碳酸化。然而，纤维外矿物质沉积的形态和优先晶体取向与天然骨不同。此外，BMC的杨氏模量和矿物质与组织的协同变形率显著低于具有相似矿物质体积分数的天然骨和部分脱矿骨。得出的结论是，虽然仿生矿化可以在BMC中实现良好的矿物质沉积量，但该材料的力学行为与天然骨不同。
