Assisted Reproductive Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong.
Li Ka Shing Institute of Health Science, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong.
J Obstet Gynaecol Res. 2020 Jun;46(6):858-863. doi: 10.1111/jog.14250. Epub 2020 Mar 18.
This aim of this study was to determine the association between uterine natural killer (uNK) cell density and chronic endometritis (CE).
Endometrial biopsies from 135 women with recurrent miscarriage were obtained precisely 7 days after luteinizing hormone surge in natural cycles. Endometrial sections were immunostained for CD56 for uNK cells and CD138 for plasma cells, respectively. Uterine NK cell counting was performed according to a standardized protocol and results were expressed as percentage of CD56+ cells/ total stromal cells. High uNK cell density was defined as >4.5% and CE was diagnosed when the plasma cell density > 5.15 cells/ 10 mm .
The uNK cells density in women with CE (median, 5.1%; range, 3.4-8.8%) was significantly (P < 0.05) higher than that of those without CE (median, 3.8%; range, 1.2%-7.3%). The prevalence of high uNK cell density in women with CE (11/29, 37.9%) was significantly (P < 0.05) higher than that of women without CE (8/106, 7.5%).
To conclude, there was a significant association between high uNK cell density and CE. In women with high uNK cell density, plasma cell should be examined to determine if the underlying cause is associated with CE.
本研究旨在确定子宫自然杀伤(uNK)细胞密度与慢性子宫内膜炎(CE)之间的关系。
在自然周期中黄体生成素激增后 7 天，从 135 例复发性流产妇女中获得子宫内膜活检。分别对子宫内膜切片进行 CD56 免疫染色以检测 uNK 细胞，和 CD138 免疫染色以检测浆细胞。按照标准化方案进行子宫 NK 细胞计数，并以 CD56+细胞/总基质细胞的百分比表示结果。高 uNK 细胞密度定义为>4.5%，当浆细胞密度>5.15 个/10mm 时诊断为 CE。
CE 患者的 uNK 细胞密度（中位数，5.1%；范围，3.4-8.8%）明显高于无 CE 患者（中位数，3.8%；范围，1.2%-7.3%）（P<0.05）。CE 患者中高 uNK 细胞密度的发生率（11/29，37.9%）明显高于无 CE 患者（8/106，7.5%）（P<0.05）。
总之，高 uNK 细胞密度与 CE 之间存在显著相关性。在 uNK 细胞密度较高的患者中，应检查浆细胞以确定潜在原因是否与 CE 相关。
