Shibasaki Y, Rønne M
Institute of Anatomy and Cytology, Odense University, Denmark.
Anticancer Res. 1988 Nov-Dec;8(6):1285-9.
Karyotypical changes in connection with spontaneous in vitro transformation of primary Chinese hamster lung cultures were followed. At the 26th passage the cultures were spontaneously transformed to an established cell line in the near-diploid range (CHAL-cells). The only chromosomal change which could be observed in the stemline of CHAL-cultures after R-banding and karyotyping was the presence of an extra chromosome giving the following karyotype, 23, XY, +mar. The distal part of the marker showed banding patterns corresponding to 3q21-3qter.
对原代中国仓鼠肺细胞培养物自发体外转化过程中的核型变化进行了跟踪。在第26代时，培养物自发转化为近二倍体范围内的一个已建立细胞系（中国仓鼠肺细胞系，CHAL细胞）。在对CHAL细胞系进行R显带和核型分析后，在其主干系中观察到的唯一染色体变化是存在一条额外的染色体，其核型为23, XY, +mar。标记染色体的远端部分显示出与3q21 - 3qter相对应的带型模式。
