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Prevalence of intestinal parasites, with emphasis on the molecular epidemiology of Giardia duodenalis and Blastocystis sp., in the Paranaguá Bay, Brazil: a community survey.巴西帕拉纳瓜湾肠道寄生虫流行情况,重点为十二指肠贾第鞭毛虫和肠孢子虫的分子流行病学:社区调查。
Parasit Vectors. 2018 Aug 30;11(1):490. doi: 10.1186/s13071-018-3054-7.
2
Genetic Diversity and Distribution of Subtype 3 in Human Populations, with Special Reference to a Rural Population in Central Mexico.遗传多样性与 3 型在人群中的分布,特别是与中美洲农村人口的关系。
Biomed Res Int. 2018 Mar 18;2018:3916263. doi: 10.1155/2018/3916263. eCollection 2018.
3
Comparison of Cytokine Responses in Ecuadorian Children Infected with , , or Both Parasites.感染 、 或两种寄生虫的厄瓜多尔儿童细胞因子反应的比较 。 (你提供的原文中寄生虫名称部分缺失,请补充完整后以便更准确理解和翻译。)
Am J Trop Med Hyg. 2017 Jun;96(6):1394-1399. doi: 10.4269/ajtmh.16-0580.
4
Blastocystis subtyping and its association with intestinal parasites in children from different geographical regions of Colombia.哥伦比亚不同地理区域儿童的芽囊原虫分型及其与肠道寄生虫的关联。
PLoS One. 2017 Feb 21;12(2):e0172586. doi: 10.1371/journal.pone.0172586. eCollection 2017.
5
Geographic distribution of human Blastocystis subtypes in South America.南美洲人芽囊原虫亚型的地理分布。
Infect Genet Evol. 2016 Jul;41:32-35. doi: 10.1016/j.meegid.2016.03.017. Epub 2016 Mar 24.
6
Effect of Poor Access to Water and Sanitation As Risk Factors for Soil-Transmitted Helminth Infection: Selectiveness by the Infective Route.获得安全饮用水和卫生设施困难作为土壤传播蠕虫感染风险因素的影响:按感染途径的选择性
PLoS Negl Trop Dis. 2015 Sep 30;9(9):e0004111. doi: 10.1371/journal.pntd.0004111. eCollection 2015 Sep.
7
Blastocystis and urticaria: Examination of subtypes and morphotypes in an unusual clinical manifestation.芽囊原虫与荨麻疹:一种不寻常临床表现中的亚型和形态型检测
Acta Trop. 2015 Aug;148:156-61. doi: 10.1016/j.actatropica.2015.05.004. Epub 2015 May 11.
8
The microbial eukaryote Blastocystis is a prevalent and diverse member of the healthy human gut microbiota.微生物真核生物芽囊原虫是健康人类肠道微生物群中一种普遍且多样的成员。
FEMS Microbiol Ecol. 2014 Oct;90(1):326-30. doi: 10.1111/1574-6941.12396. Epub 2014 Sep 15.
9
A novel, multi-parallel, real-time polymerase chain reaction approach for eight gastrointestinal parasites provides improved diagnostic capabilities to resource-limited at-risk populations.一种新型的、多平行的、实时聚合酶链反应方法可用于检测八种胃肠道寄生虫,为资源有限的高危人群提供了更好的诊断能力。
Am J Trop Med Hyg. 2013 Jun;88(6):1041-7. doi: 10.4269/ajtmh.12-0726. Epub 2013 Mar 18.
10
New insights into Blastocystis spp.: a potential link with irritable bowel syndrome.对芽囊原虫属的新见解:与肠易激综合征的潜在联系。
PLoS Pathog. 2012;8(3):e1002545. doi: 10.1371/journal.ppat.1002545. Epub 2012 Mar 15.

多平行实时定量 PCR 在洪都拉斯农村地区检测和其他胃肠道寄生虫感染关联的应用。

Use of Multi-Parallel Real-Time Quantitative PCR to Determine Prevalence and Association with Other Gastrointestinal Parasite Infection in a Rural Honduran Location.

机构信息

Laboratory of Clinical Parasitology and Diagnostics, National School of Tropical Medicine, Baylor College of Medicine, Houston, Texas.

Instituto de Investigaciones en Microbiología, Universidad Nacional Autónoma de Honduras, Tegucigalpa, Honduras.

出版信息

Am J Trop Med Hyg. 2020 Jun;102(6):1373-1375. doi: 10.4269/ajtmh.19-0876.

DOI:10.4269/ajtmh.19-0876
PMID:32189609
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7253141/
Abstract

To determine whether the presence of is associated with other gastrointestinal parasite infections, stool samples from 95 Honduran rural children were analyzed using multi-parallel quantitative real-time polymerase chain reaction (PCR) and Kato-Katz. Combined results detected the following prevalence: , 71.6%; , 63.2%; , 40.0%; , 15.8%; and , 4.2%. Age was found associated with the quantity of both DNA ( = 0.524, < 0.001) and DNA in the stool (fg/µL) by quantitative PCR ( = 0.272, < 0.001). In addition, there was an association with and infection (odds ratio [OR] = 4.72; 95% CI = 1.83, 12.20; < 0.001). These findings demonstrate a high prevalence of and other intestinal parasites in a rural location in Honduras.

摘要

为了确定 是否与其他胃肠道寄生虫感染有关,我们使用多平行定量实时聚合酶链反应(PCR)和加藤法对 95 名洪都拉斯农村儿童的粪便样本进行了分析。综合结果显示以下流行率: ,71.6%; ,63.2%; ,40.0%; ,15.8%; 和 ,4.2%。通过定量 PCR 发现年龄与 DNA( = 0.524, < 0.001)和粪便中 DNA(fg/µL)的数量有关( = 0.272, < 0.001)。此外, 与 和 感染之间存在关联(比值比[OR] = 4.72;95%置信区间[CI] = 1.83, 12.20; < 0.001)。这些发现表明在洪都拉斯的农村地区存在 和其他肠道寄生虫的高流行率。