多平行实时定量 PCR 在洪都拉斯农村地区检测和其他胃肠道寄生虫感染关联的应用。
Use of Multi-Parallel Real-Time Quantitative PCR to Determine Prevalence and Association with Other Gastrointestinal Parasite Infection in a Rural Honduran Location.
机构信息
Laboratory of Clinical Parasitology and Diagnostics, National School of Tropical Medicine, Baylor College of Medicine, Houston, Texas.
Instituto de Investigaciones en Microbiología, Universidad Nacional Autónoma de Honduras, Tegucigalpa, Honduras.
出版信息
Am J Trop Med Hyg. 2020 Jun;102(6):1373-1375. doi: 10.4269/ajtmh.19-0876.
Abstract
To determine whether the presence of is associated with other gastrointestinal parasite infections, stool samples from 95 Honduran rural children were analyzed using multi-parallel quantitative real-time polymerase chain reaction (PCR) and Kato-Katz. Combined results detected the following prevalence: , 71.6%; , 63.2%; , 40.0%; , 15.8%; and , 4.2%. Age was found associated with the quantity of both DNA ( = 0.524, < 0.001) and DNA in the stool (fg/µL) by quantitative PCR ( = 0.272, < 0.001). In addition, there was an association with and infection (odds ratio [OR] = 4.72; 95% CI = 1.83, 12.20; < 0.001). These findings demonstrate a high prevalence of and other intestinal parasites in a rural location in Honduras.
摘要
为了确定 是否与其他胃肠道寄生虫感染有关，我们使用多平行定量实时聚合酶链反应（PCR）和加藤法对 95 名洪都拉斯农村儿童的粪便样本进行了分析。综合结果显示以下流行率： ，71.6%； ，63.2%； ，40.0%； ，15.8%； 和 ，4.2%。通过定量 PCR 发现年龄与 DNA（ = 0.524， < 0.001）和粪便中 DNA（fg/µL）的数量有关（ = 0.272， < 0.001）。此外， 与 和 感染之间存在关联（比值比[OR] = 4.72；95%置信区间[CI] = 1.83, 12.20； < 0.001）。这些发现表明在洪都拉斯的农村地区存在 和其他肠道寄生虫的高流行率。
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