Shultz T D, Santamaria A G, Gridley D S, Stickney D R, Slater J M
Department of Biochemistry, School of Medicine, Loma Linda University, CA 92350.
Anticancer Res. 1988 Nov-Dec;8(6):1313-8.
The in vitro effect of vitamin B-6 supplementation on the growth of a human malignant melanoma cell line (M21-HPB) was investigated. Varying concentrations of pyridoxine (PN) or pyridoxal (PL) were added to cell cultures and incubated for 12 days. Pharmacologic levels of PL (0.25-0.5 mM) resulted in significant reductions in cell proliferation. Physiologic levels (0.005 mM) did not retard growth. Cells incubated with PN showed growth stimulation. Intracellular levels of PL and pyridoxal 5'-phosphate (PLP) were increased in cells exposed to pharmacologic PL (0.05-0.5 mM) concentrations, but not PN. These data suggest that PL or PLP may be involved in regulating the growth of melanoma cells and that vitamin B-6 may have potential use as an antineoplastic agent.
研究了补充维生素B-6对人恶性黑色素瘤细胞系（M21-HPB）生长的体外作用。将不同浓度的吡哆醇（PN）或吡哆醛（PL）添加到细胞培养物中，并孵育12天。药理水平的PL（0.25 - 0.5 mM）导致细胞增殖显著减少。生理水平（0.005 mM）并未阻碍生长。用PN孵育的细胞显示出生长刺激。暴露于药理PL（0.05 - 0.5 mM）浓度的细胞中，PL和磷酸吡哆醛（PLP）的细胞内水平升高，但PN处理的细胞未出现这种情况。这些数据表明，PL或PLP可能参与调节黑色素瘤细胞的生长，并且维生素B-6可能具有作为抗肿瘤药物的潜在用途。
