Fechner G, Petkovits T, Brinkmann B
Institut für Rechtsmedizin, Westfälischen Wilhelms-Universität Münster.
Arch Kriminol. 1988 Nov-Dec;182(5-6):154-8.
Post mortem tissue samples from various human organs (skin, muscle, liver, kidney, brain) were stored at room temperature for a period of one year. During this time the samples were regularly histologically tested. Without any pretreatment of the samples a histological preparation and identification was no longer possible after only a few hours. By the use of a rehydration solution consisting of absolute ethanol (30 ml), aqua dest. (50 ml) and 0.2 M phosphate buffer pH 7.0 (20 ml) a reconstitution and identification was again possible. Further immunhistochemical examinations were also unaffected by the solution. The use of microwave stimulation allowed a reduction of the rehydration process from 24 hours to only 4 hours.
取自各种人体器官（皮肤、肌肉、肝脏、肾脏、大脑）的尸检组织样本在室温下保存了一年。在此期间，定期对样本进行组织学检测。在未对样本进行任何预处理的情况下，仅数小时后就无法再进行组织学制片和鉴定。通过使用由无水乙醇（30毫升）、蒸馏水（50毫升）和0.2M pH 7.0的磷酸盐缓冲液（20毫升）组成的复水溶液，又能够进行重构和鉴定了。进一步的免疫组织化学检查也不受该溶液影响。使用微波刺激可将复水过程从24小时缩短至仅4小时。
