Njan Anoka A, Ologe Mary O, Olorundare Olufunke E, Afolabi Saheed O, Ejimkonye Benjamin C, Olaoye Solomon O, Fatigun Chloe O, Akinola Olugbenga, Soje Anthonia, Erdogan Ozlem Nazan, Asogwa Nnaemeka, Iwalewa Olugbenga E
Department of Pharmacology and Therapeutic, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin, Ilorin, Nigeria.
General Hospital, Kishi, Oyo State, Nigeria.
Heliyon. 2020 Mar 12;6(3):e03514. doi: 10.1016/j.heliyon.2020.e03514. eCollection 2020 Mar.
(camphor) {CHO} is a chemical substance used mostly amongst the Yoruba ethnic group in Western Nigeria to treat infantile colic during early childhood. This study assess the neurotoxic potentials of following sub-chronic exposure in female albino Wistar rats.
Twenty-eight female rats (mean weight of 130 g) were randomly selected and assigned into four (4) groups. Control, received 1ml coconut oil while the treatment groups received 79, 158 and 237. mg/kg b.wt (d ose p.o) of for the period of 14 days. On day fifteen, animals were dissected and the brain organ excised for the homogenate and histopathologic assay, blood samples were also collected for haematological analysis. Morris Water Maze experiment for reference memory was also carried out to ascertain effect of in the Central Nervous system (CNS).
A trend toward decreased body-weight gain and increase brain weight was observed in -treated rats but was statistically not significant, compared to control. The biochemical assessment of the antioxidant status of brains of -treated rats showed significant (p ≤ 0.05) increase in activities of some anti-oxidant enzymes (Superoxide dismutase (SOD), Glutathione peroxide (GPx), and Catalase (CAT)). There was increase in acetylcholinesterase (AChE), Malondialdehyde (MDA), and Total protein activities in the brain of treated rats compared to control. Alterations of the haematological parameters were observed, with the plasma granulocytes, lymphocytes, and haemoglobin (HGB), showing significant decrease in the treated rats compared to control. The water maze test showed a marked increase in spatial learning and memory time (seconds) in -treated rats, compared to control and across treated groups.
The present study provides indication that shows apparent neurotoxicity in experimental animals. Incessant exposure in humans though may lead to development of some central nervous system defects.
（樟脑）{CHO}是一种化学物质，在尼日利亚西部的约鲁巴族中主要用于治疗幼儿期的婴儿腹绞痛。本研究评估了雌性白化Wistar大鼠亚慢性暴露于以下物质后的神经毒性潜力。
随机选择28只雌性大鼠（平均体重130克），分为四组。对照组给予1毫升椰子油，而治疗组在14天内分别给予79、158和237毫克/千克体重（口服剂量）的（物质名称未明确给出）。在第15天，解剖动物并切除脑器官进行匀浆和组织病理学分析，同时采集血样进行血液学分析。还进行了莫里斯水迷宫实验以评估（物质名称未明确给出）对中枢神经系统（CNS）的影响，以确定其对参考记忆的作用。
与对照组相比，接受（物质名称未明确给出）处理的大鼠体重增加有下降趋势，脑重增加，但差异无统计学意义。对接受（物质名称未明确给出）处理的大鼠脑抗氧化状态的生化评估显示，一些抗氧化酶（超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT））的活性显著增加（p≤0.05）。与对照组相比，接受（物质名称未明确给出）处理的大鼠脑内乙酰胆碱酯酶（AChE）、丙二醛（MDA）和总蛋白活性增加。观察到血液学参数发生改变，与对照组相比，处理组大鼠的血浆粒细胞、淋巴细胞和血红蛋白（HGB）显著减少。水迷宫试验显示，与对照组和各处理组相比，接受（物质名称未明确给出）处理的大鼠空间学习和记忆时间（秒）显著增加。
本研究表明（物质名称未明确给出）在实验动物中表现出明显的神经毒性。尽管人类持续接触可能会导致一些中枢神经系统缺陷的发生。
