State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing, 210009, P. R. China.
Chem Biodivers. 2020 May;17(5):e2000056. doi: 10.1002/cbdv.202000056. Epub 2020 Apr 28.
Osteoporosis (OP) is a metabolic bone disease affecting nearly 200 million individuals globally. Morinda officinalis F.C.How (MOH) has long been used as a traditional herbal medicine for the treatment of bone fractures and joint diseases in China. However, it still remains unclear how the compounds in MOH work synergistically for treating OP. In this study, we used prednisolone (PNSL)-induced zebrafish OP model to screen the antiosteoporosis components in MOH. A network pharmacology approach was further proposed to explore the underlying mechanism of MOH on OP. The PNSL-induced zebrafish model validated that two anthraquinones, one iridoid glycoside, and two saccharides exerted antiosteoporotic effect. We constructed the components-targets network and obtained the enriched Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. A total of 26 candidate compounds of MOH and 257 related targets could probably treat OP through regulating osteoclast differentiation and MAPK signaling pathway. Our work developed a strategy to screen the antiosteoporosis components and explore the underlying mechanism of MOH for treating OP at a network pharmacology level.
骨质疏松症（OP）是一种影响全球近 2 亿人的代谢性骨病。桑科植物巴戟天（MOH）在中国一直被用作治疗骨折和关节疾病的传统草药。然而，MOH 中的化合物如何协同作用治疗 OP 仍不清楚。在这项研究中，我们使用泼尼松龙（PNSL）诱导的斑马鱼 OP 模型筛选 MOH 中的抗骨质疏松成分。进一步提出了一种网络药理学方法来探索 MOH 对 OP 的潜在作用机制。PNSL 诱导的斑马鱼模型验证了两种蒽醌、一种环烯醚萜苷和两种糖具有抗骨质疏松作用。我们构建了成分-靶点网络，并获得了富集的基因本体论（GO）术语和京都基因与基因组百科全书（KEGG）途径。MOH 的 26 种候选化合物和 257 个相关靶点可能通过调节破骨细胞分化和 MAPK 信号通路来治疗 OP。我们的工作开发了一种在网络药理学水平上筛选抗骨质疏松成分和探索 MOH 治疗 OP 的潜在作用机制的策略。
