Le Nhan, Subhash Hrebesh M, Kilpatrick-Liverman LaTonya, Wang Ruikang K
Department of Bioengineering, University of Washington, Seattle, WA, USA.
Clinical Method Development - Oral Care, Colgate-Palmolive Company, Piscataway, NJ, USA.
J Biophotonics. 2020 Jul;13(7):e202000026. doi: 10.1002/jbio.202000026. Epub 2020 Mar 25.
We report the development of an integrated multifunctional imaging system capable of providing anatomical (optical coherence tomography, OCT), functional (OCT angiography, OCTA) and molecular imaging (light-induced autofluorescence, LIAF) for in vivo dental applications. Blue excitation light (405 nm) was used for LIAF imaging, while the OCT was powered by a 1310 nm swept laser source. A red-green-blue digital camera, with a 450 nm cut-on broadband optical filter, was used for LIAF detection. The exciting light source and camera were integrated directly with the OCT scanning probe. The integrated system used two noninvasive imaging modalities to improve the speed of in vivo OCT data collection and to better target the regions of interest. The newly designed system maintained the ability to detect differences between healthy and hypomineralized teeth, identify dental biofilm and visualize the microvasculature of gingival tissue. The development of the integrated OCT-LIAF system provides an opportunity to conduct clinical studies more efficiently, examining changes in oral conditions over time.
我们报告了一种集成多功能成像系统的开发，该系统能够为体内牙科应用提供解剖学成像（光学相干断层扫描，OCT）、功能成像（OCT血管造影，OCTA）和分子成像（光诱导自发荧光，LIAF）。蓝色激发光（405 nm）用于LIAF成像，而OCT由1310 nm扫频激光源驱动。配备450 nm截止宽带光学滤光片的红绿蓝数码相机用于LIAF检测。激发光源和相机直接与OCT扫描探头集成。该集成系统使用两种非侵入性成像方式来提高体内OCT数据采集的速度，并更好地靶向感兴趣区域。新设计的系统保持了检测健康牙齿和矿化不足牙齿之间差异、识别牙菌斑以及可视化牙龈组织微血管系统的能力。集成OCT-LIAF系统的开发为更高效地开展临床研究提供了机会，可随时间检查口腔状况的变化。
