Laboratory of Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University, Daegu, Korea.
Institute of Traditional Medicine and Bioscience, Daejeon University, Daejeon, Korea.
J Med Food. 2020 Mar;23(3):312-318. doi: 10.1089/jmf.2019.4625.
The aim of this study was to investigate the efficacy of an ethanol extract of (PA) and its mechanistic pathway of action at the molecular level for its antiobesity properties. Four-week old male Institute of Cancer Research (ICR) mice were acclimatized for a week before starting the high-fat diet (HFD) for 2 weeks to induce obesity, followed by 8 more weeks of oral administration of 10 mg/kg orlistat and 300 mg/kg of PA extract, along with HFD. Body weights of the mice and feed and water intake were recorded weekly. After a total of 12 weeks, mice were euthanized, and blood, liver, and adipose tissues were harvested for further analysis. Administration of PA extract inhibited the progression of obesity by reducing weight gain, weight of adipose tissue, and normalizing serum triglyceride, glucose, total cholesterol, high-density lipoprotein, low-density lipoprotein, alanine aminotransferase, and aspartate aminotransferase. PA extract prevented the progression of nonalcoholic steatohepatitis induced by HFD and prevented the enlargement of liver. Phosphorylation of adenosine monophosphate-activated protein kinase increased while phosphorylation of acetyl-CoA carboxylase was reduced. The browning gene uncoupling protein 1 expression was also increased by PA extract treatment. Our findings revealed that the antiobesity properties of PA extract may be mediated by browning of white adipose tissue.
本研究旨在探讨（PA）的乙醇提取物的功效及其在分子水平上的作用机制，以研究其抗肥胖特性。将 4 周龄雄性癌症研究所（ICR）小鼠适应环境一周，然后开始高脂饮食（HFD）2 周，以诱导肥胖，随后再进行 8 周的 10mg/kg 奥利司他和 300mg/kg PA 提取物的口服给药，同时给予 HFD。每周记录小鼠的体重、饲料和水的摄入量。总共 12 周后，对小鼠进行安乐死，并采集血液、肝脏和脂肪组织进行进一步分析。PA 提取物通过减少体重增加、脂肪组织重量和使血清甘油三酯、葡萄糖、总胆固醇、高密度脂蛋白、低密度脂蛋白、丙氨酸氨基转移酶和天冬氨酸氨基转移酶正常化来抑制肥胖的进展。PA 提取物可防止 HFD 诱导的非酒精性脂肪性肝炎的进展，并防止肝脏肿大。磷酸化的一磷酸腺苷激活蛋白激酶增加，而乙酰辅酶 A 羧化酶的磷酸化减少。PA 提取物处理还增加了解偶联蛋白 1 的表达。我们的研究结果表明，PA 提取物的抗肥胖特性可能是通过白色脂肪组织的褐变来介导的。
