Lubiński J, Chosia M, Huebner K
Department of Tumor Pathology, Medical Academy, Szczecin, Poland.
Anal Quant Cytol Histol. 1988 Dec;10(6):391-8.
The configurations of immunoglobulin genes, T-cell receptor (TCR) beta chain genes and bcl-2 genes were analyzed by Southern blotting in DNAs derived from 35 fine needle aspiration biopsies from various lymphoproliferative disorders. Only 1 of 16 benign lymphoproliferative disorders showed clonality: the lymph node of a patient with Wiskott-Aldrich immunodeficiency syndrome, in which clonal rearrangement of the TCR beta chain gene was detected. Clonality was demonstrated in all 14 non-Hodgkin's lymphomas (NHLs), 2 of 3 cases of Hodgkin's disease (HD) and 2 cases diagnosed as NHL or angioimmunoblastic lymphadenopathy (AILD). None of the aspirates exhibited rearrangement of the bcl-2 gene. The studies of diagnostically difficult cases proved that molecular genetic analysis of DNA, when appropriately combined with clinical data and light microscopic analysis of the lesions, can be helpful in distinguishing between: (1) a hyperplastic lymph node and NHL or AILD; (2) NHL and well-differentiated lymphocytes; and (3) a hyperplastic lymph node and HD.
通过Southern印迹法分析了来自35例各种淋巴增生性疾病细针穿刺活检样本的DNA中的免疫球蛋白基因、T细胞受体（TCR）β链基因和bcl-2基因的构型。16例良性淋巴增生性疾病中只有1例显示出克隆性：1例患有威斯科特-奥尔德里奇免疫缺陷综合征患者的淋巴结，其中检测到TCRβ链基因的克隆重排。在所有14例非霍奇金淋巴瘤（NHL）、3例霍奇金病（HD）中的2例以及2例诊断为NHL或血管免疫母细胞性淋巴结病（AILD）的病例中均证实有克隆性。所有穿刺样本均未显示bcl-2基因重排。对诊断困难病例的研究证明，DNA的分子遗传学分析若能与临床数据及病变的光镜分析适当结合，有助于鉴别：（1）增生性淋巴结与NHL或AILD；（2）NHL与高分化淋巴细胞；（3）增生性淋巴结与HD。
