ACS Appl Mater Interfaces. 2021 Sep 8;13(35):41405-41413. doi: 10.1021/acsami.0c00434. Epub 2020 Mar 19.
In situ quantification and imaging of low-level intracellular microRNAs (miRs) are important areas in biosensor research. Herein, DNA-driven FeCuSe@upconversion nanoparticle (UCNP) core@satellite nanostructures were developed to probe microRNA-21 (miR-21). FeCuSe@UCNP probes displayed dual signals: upconversion luminescence (UCL) and magnetic resonance imaging (MRI). In the presence of miR-21, the luminescence signal was restored and the value was significantly increased because of dissociation of UCNPs from the assemblies. There was a good linear relationship between the dual signals and the expression levels of miR-21 in the range of 0.035-31.824 amol/ng. The limit of detection (LOD) was 0.0058 amol/ng for the luminescence intensity and 0.0182 amol/ng for the MRI signal. This method opens a new avenue for intracellular miR-21 detection with high sensitivity and specificity.
在生物传感器研究中，对低水平细胞内 microRNA（miRs）进行原位定量和成像分析是一个重要的领域。本文构建了 DNA 驱动的 FeCuSe@上转换纳米粒子（UCNP）核@卫星纳米结构，用于探测 microRNA-21（miR-21）。FeCuSe@UCNP 探针表现出双信号：上转换发光（UCL）和磁共振成像（MRI）。在 miR-21 存在的情况下，由于 UCNPs 从组装体中解离，发光信号得到恢复， 值显著增加。双信号与 miR-21 的表达水平之间存在良好的线性关系，其范围为 0.035-31.824 amol/ng。发光强度的检测限（LOD）为 0.0058 amol/ng，MRI 信号的检测限为 0.0182 amol/ng。该方法为具有高灵敏度和特异性的细胞内 miR-21 检测开辟了新途径。
