Mingala Claro N., Pasag Alma Corazon P., Salinas Marvin Bryan S., Balbin Michelle M., Villanueva Marvin A.
College of Veterinary Science and Medicine, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines
Biosafety and Environment Section, Philippine Carabao Center National Headquarters and Gene Pool, Science City of Munoz 3120, Nueva Ecija, Philippines
Ann Parasitol. 2019;65(4):381–386. doi: 10.17420/ap6504.224.
This study detected and characterized the TevAT1 gene of Trypanosoma evansi isolates from Philippine water buffaloes (Bubalus bubalis). A total of 68 blood samples from Philippine water buffaloes were subjected to DNA extraction and PCR assay was performed using RoTat 1.2 gene to detect T. evansi. Those samples positive for T. evansi subsequently underwent another PCR assay to detect the presence of TevAT1 gene. Trypanosoma evansi was detected in 26.47% (18/68) blood samples in which distributed throughout the main islands of the country (4 from Luzon, 2 from Visayas and 12 from Mindanao). However, only 10 of these samples were positive for TevAT1 gene. Sequence alignment of the TevAT1 gene from local isolates showed no single nucleotide polymorphisms when compared to other strains in various countries. Those T. evansi without the gene of interest could be possibly resistant to some trypanocidal drugs but this needs to be further investigated in-vitro or in-vivo.
本研究对来自菲律宾水牛（Bubalus bubalis）的伊氏锥虫分离株的TevAT1基因进行了检测和特征分析。从菲律宾水牛身上采集了总共68份血样进行DNA提取，并使用RoTat 1.2基因进行PCR检测以检测伊氏锥虫。那些伊氏锥虫检测呈阳性的样本随后进行了另一项PCR检测以检测TevAT1基因的存在。在26.47%（18/68）的血样中检测到了伊氏锥虫，这些血样分布在该国的主要岛屿（吕宋岛4份、米沙鄢群岛2份、棉兰老岛12份）。然而，这些样本中只有10份TevAT1基因检测呈阳性。与各国的其他菌株相比，本地分离株的TevAT1基因序列比对未显示单核苷酸多态性。那些没有目标基因的伊氏锥虫可能对某些杀锥虫药物具有抗性，但这需要在体外或体内进一步研究。
