Teubner Marlene, Lenzen Benjamin, Espenberger Lucas Bernal, Fuss Janina, Nickelsen Jörg, Krause Kirsten, Ruwe Hannes, Schmitz-Linneweber Christian
Institute of Biology, Department of Life Sciences, Humboldt University Berlin, 10115 Berlin, Germany.
Department of Arctic and Marine Biology, UiT The Arctic University of Norway, Framstredet 39, 9019 Tromsø, Norway.
Plants (Basel). 2020 Mar 17;9(3):367. doi: 10.3390/plants9030367.
Chloroplast RNAs are stabilized and processed by a multitude of nuclear-encoded RNA-binding proteins, often in response to external stimuli like light and temperature. A particularly interesting RNA-based regulation occurs with the mRNA, which shows light-dependent translation. Recently, the chloroplast ribonucleoprotein CP33B was identified as a ligand of the mRNA. We here characterized the interaction of CP33B with chloroplast RNAs in greater detail using a combination of RIP-chip, quantitative dot-blot, and RNA-Bind-n-Seq experiments. We demonstrate that CP33B prefers over all other chloroplast RNAs and associates with the vast majority of the transcript pool. The RNA sequence target motif, determined in vitro, does not fully explain CP33B's preference for , suggesting that there are other determinants of specificity in vivo.
叶绿体RNA由多种核编码的RNA结合蛋白稳定并加工，这通常是对光和温度等外部刺激的响应。一种特别有趣的基于RNA的调控发生在mRNA上，它表现出光依赖性翻译。最近，叶绿体核糖核蛋白CP33B被鉴定为该mRNA的配体。我们在这里使用RIP芯片、定量点杂交和RNA-Bind-n-Seq实验相结合的方法，更详细地表征了CP33B与叶绿体RNA的相互作用。我们证明，CP33B相对于所有其他叶绿体RNA更倾向于结合该mRNA，并与绝大多数该转录本池相关联。体外确定的RNA序列靶基序并不能完全解释CP33B对该mRNA的偏好，这表明体内存在其他特异性决定因素。
